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Proceedings Paper

Amperometric biosensors for the determination of heavy metals
Author(s): Dario Compagnone; Giuseppe Palleschi; Giuseppe Varallo; PierLuigi Imperiali
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Paper Abstract

A bioelectrochemical method for the determination of heavy metal ions has been developed. This method is based on the inhibition effect of metal ions on the enzymatic activity of oxidase enzymes. The enzymatic activity was determined with an amperometric hydrogen peroxide probe. The inhibition effect on enzymes in solution and covalently immobilized on polymeric supports has been evaluated. Hg(II) was the metal ion that inhibited almost all the enzymes, particularly glycerol-3-P oxidase. Hg(II) was detected in the 0.05/0.5 ppm range with the enzyme in solution. Calibration curves for Hg(II) were also obtained with the other oxidase enzymes in the 0.5/10 ppm range. The other metal ions tested inhibited the enzymes more specifically. The metal ion/enzyme systems which gave the best inhibition were Se(IV)/glutathione oxidase, Ni(II)/sarcosine oxidase, V(V)/glutathione oxidase, Cu(II)/alcohol oxidase from Pichia Pastoris and Cd(II)/D-aminoacid oxidase. All these metal ions were detected in the 0.1/10 ppm range using the enzymes in solution or covalently immobilized.

Paper Details

Date Published: 9 October 1995
PDF: 12 pages
Proc. SPIE 2504, Environmental Monitoring and Hazardous Waste Site Remediation, (9 October 1995); doi: 10.1117/12.224096
Show Author Affiliations
Dario Compagnone, Univ. di Napoli Federico II (Italy)
Giuseppe Palleschi, Univ. di Napoli Federico II (Italy)
Giuseppe Varallo, ENEL (Italy)
PierLuigi Imperiali, ISMES SpA (Italy)

Published in SPIE Proceedings Vol. 2504:
Environmental Monitoring and Hazardous Waste Site Remediation
Tuan Vo-Dinh, Editor(s)

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