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A comparison of classical histology to anatomy revealed by hard x-rays
Author(s): Claus-Peter Richter; Xiaodong Tan; Hunter Young; Stuart Stock; Alan Robinson; Orest Byskosh; Jing Zheng; Carmen Soriano; Xianghui Xiao; Donna Whitlon
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Paper Abstract

Many diseases trigger morphological changes in affected tissue. Today, classical histology is still the “gold standard” used to study and describe those changes. Classical histology, however, is time consuming and requires chemical tissue manipulations that can result in significant tissue distortions. It is sometimes difficult to separate tissue-processing artifacts from changes caused by the disease process. We show that synchrotron X-ray phase-contrast micro-computed tomography (micro-CT) can be used to examine non-embedded, hydrated tissue at a resolution comparable to that obtained with classical histology. The data analysis from stacks of reconstructed micro-CT images is more flexible and faster than when using the classical, physically embedded sections that are by necessity fixed in a particular orientation. We show that in a three-dimensional (3D) structure with meticulous structural details such as the cochlea and the kidney, micro-CT is more flexible, faster and more convenient for morphological studies and disease diagnoses.

Paper Details

Date Published: 3 October 2016
PDF: 7 pages
Proc. SPIE 9967, Developments in X-Ray Tomography X, 99671I (3 October 2016); doi: 10.1117/12.2239146
Show Author Affiliations
Claus-Peter Richter, Northwestern Univ. (United States)
Xiaodong Tan, Northwestern Univ. (United States)
Hunter Young, Northwestern Univ. (United States)
Stuart Stock, Northwestern Univ. (United States)
Alan Robinson, Northwestern Univ. (United States)
Orest Byskosh, Northwestern Univ. (United States)
Jing Zheng, Northwestern Univ. (United States)
Carmen Soriano, Argonne National Lab. (United States)
Xianghui Xiao, Argonne National Lab. (United States)
Donna Whitlon, Northwestern Univ. (United States)


Published in SPIE Proceedings Vol. 9967:
Developments in X-Ray Tomography X
Stuart R. Stock; Bert Müller; Ge Wang, Editor(s)

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