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Proceedings Paper

Phase-contrast tomography of neuronal tissues: from laboratory- to high resolution synchrotron CT
Author(s): Mareike Töpperwien; Martin Krenkel; Kristin Müller; Tim Salditt
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Paper Abstract

Assessing the three-dimensional architecture of neuronal tissues with sub-cellular resolution presents a significant analytical challenge. Overcoming the limitations associated with serial slicing, phase-contrast x-ray tomography has the potential to contribute to this goal. Even compact laboratory CT at an optimized liquid-metal jet micro- focus source combined with suitable phase-retrieval algorithms and preparation protocols can yield renderings with single cell sensitivity in millimeter sized brain areas of mouse. Here, we show the capabilities of the setup by imaging a Golgi-Cox impregnated mouse brain. Towards higher resolution we extend these studies at our recently upgraded waveguide-based cone-beam holo-tomography instrument GINIX at DESY. This setup allows high resolution recordings with adjustable field of view and resolution, down to the voxel sizes in the range of a few ten nanometers. The recent results make us confident that important issues of neuronal connectivity can be addressed by these methods, and that 3D (virtual) histology with nanoscale resolution will become an attractive modality for neuroscience research.

Paper Details

Date Published: 3 October 2016
PDF: 16 pages
Proc. SPIE 9967, Developments in X-Ray Tomography X, 99670T (3 October 2016); doi: 10.1117/12.2238496
Show Author Affiliations
Mareike Töpperwien, Georg-August-Univ. Göttingen (Germany)
Martin Krenkel, Georg-August-Univ. Göttingen (Germany)
Kristin Müller, Georg-August-Univ. Göttingen (Germany)
Tim Salditt, Georg-August-Univ. Göttingen (Germany)

Published in SPIE Proceedings Vol. 9967:
Developments in X-Ray Tomography X
Stuart R. Stock; Bert Müller; Ge Wang, Editor(s)

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