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Proceedings Paper

High accuracy indirect optical manipulation of live cells with functionalized microtools
Author(s): Gaszton Vizsnyiczai; Badri L. Aekbote; András Buzás; István Grexa; Pál Ormos; Lóránd Kelemen
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Paper Abstract

Optical micro manipulation of live cells has been extensively used to study a wide range of cellular phenomena with relevance in basic research or in diagnostics. The approaches span from manipulation of many cells for high throughput measurement or sorting, to more elaborated studies of intracellular events on trapped single cells when coupled with modern imaging techniques. In case of direct cell trapping the damaging effects of light-cell interaction must be minimized, for instance with the choice of proper laser wavelength. Microbeads have already been used for trapping cells indirectly thereby reducing the irradiation damage and increasing trapping efficiency with their high refractive index contrast. We show here that such intermediate objects can be tailor-made for indirect cell trapping to further increase cell-to-focal spot distance while maintaining their free and fast maneuverability. Carefully designed structures were produced with two-photon polymerization with shapes optimized for effective manipulation and cell attachment. Functionalization of the microstructures is also presented that enables cell attachment to them within a few seconds with strength much higher that the optical forces. Fast cell actuation in 6 degrees of freedom is demonstrated with the outlook to possible applications in cell imaging.

Paper Details

Date Published: 16 September 2016
PDF: 7 pages
Proc. SPIE 9922, Optical Trapping and Optical Micromanipulation XIII, 992216 (16 September 2016); doi: 10.1117/12.2237601
Show Author Affiliations
Gaszton Vizsnyiczai, Sapienza Univ. di Roma (Italy)
Badri L. Aekbote, Ulsan National Institute of Science and Technology (Korea, Republic of)
András Buzás, Institute of Biophysics, Biological Research Ctr. (Hungary)
István Grexa, Institute of Biophysics (Hungary)
Pál Ormos, Institute of Biophysics, Biological Research Ctr. (Hungary)
Lóránd Kelemen, Institute of Biophysics (Hungary)

Published in SPIE Proceedings Vol. 9922:
Optical Trapping and Optical Micromanipulation XIII
Kishan Dholakia; Gabriel C. Spalding, Editor(s)

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