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Time-resolved and polarised microspectroscopy of thin films of bio- and nanomaterials
Author(s): Hamid Soleimaninejad; Yuning Hong; Trevor A. Smith
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Paper Abstract

We report on the use of time-resolved and polarised evanescent wave-induced fluorescence anisotropy measurements to probe molecular photophysics, motion and energy migration of fluorescent species in close proximity to a silica/film interface. In particular we show that the fluorescence decay and anisotropy of common fluorophores varies as a function of the plane of the fluorophore with respect to the interface, the distance from the interface, and as a function of position (using polarised EW imaging). We have applied time-resolved and polarised EW-induced fluorescence microspectroscopic measurements to dyes, thin polymer nanoparticle films and cells on silica surfaces, probing the variation in the photophysical dynamics within the films.

Paper Details

Date Published: 12 October 2016
PDF: 9 pages
Proc. SPIE 9923, Physical Chemistry of Interfaces and Nanomaterials XV, 99230M (12 October 2016); doi: 10.1117/12.2237084
Show Author Affiliations
Hamid Soleimaninejad, The Univ. of Melbourne (Australia)
Yuning Hong, The Univ. of Melbourne (Australia)
Trevor A. Smith, The Univ. of Melbourne (Australia)


Published in SPIE Proceedings Vol. 9923:
Physical Chemistry of Interfaces and Nanomaterials XV
Artem A. Bakulin; Robert Lovrincic; Natalie Banerji, Editor(s)

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