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Proceedings Paper

A linear algorithm for quantitative measure of corneal collagen fiber orientation using second harmonic generation microscopy
Author(s): James McLean; Charles DiMarzio
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Paper Abstract

There is significant interest in the scientific community to develop a reliable and precise quantification of the direction of collagen fibers within the cornea in order to learn more about how collagen contributes to the cornea's shape and structure. Previous work has shown that quantification of these fibers' orientations is possible using Second Harmonic Generation (SHG) microscopy, a modality that utilizes the non-centrosymmetric properties of collagen to obtain details of their macromolecular structure. Many attempts at using SHG to this purpose result in whole volume approximations which do not take into account variations through the depth of the cornea. Additionally, other algorithms have used non-linear processes which limit computation time and result in sensitivity to sample area, resolution, and illumination. We propose a linear method for quantification of collagen fiber orientation which utilizes the precise sectioning properties of SHG and is independent of non-linear artifacts to aid in further classification of cornea structure.

Paper Details

Date Published: 15 March 2016
PDF: 7 pages
Proc. SPIE 9713, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXIII, 971317 (15 March 2016); doi: 10.1117/12.2235289
Show Author Affiliations
James McLean, Northeastern Univ. (United States)
Charles DiMarzio, Northeastern Univ. (United States)


Published in SPIE Proceedings Vol. 9713:
Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXIII
Thomas G. Brown; Carol J. Cogswell; Tony Wilson, Editor(s)

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