Share Email Print
cover

Proceedings Paper

SDS-binding assay based on tyrosine fluorescence as a tool to determine binding properties of human serum albumin in blood plasma
Author(s): Nadezda Zhdanova; Evgeny Shirshin; Victor Fadeev; Alexander Priezzhev
Format Member Price Non-Member Price
PDF $14.40 $18.00

Paper Abstract

Among all plasma proteins human serum albumin (HSA) is the most studied one as it is the main transport protein and can bind a wide variety of ligands especially fatty acids (FAs). The concentration of FAs bound to HSA in human blood plasma differs by three times under abnormal conditions (fasting, physical exercises or in case of social important diseases). In the present study a surfactant sodium dodecyl sulfate (SDS) was used to simulate FAs binding to HSA. It was shown that the increase of Tyr fluorescence of human blood plasma due to SDS addition can be completely explained by HSA-SDS complex formation. Binding parameters of SDS-HSA complex (average number of sites and apparent constant of complex formation) were determined from titration curves based on tyrosine (Tyr) fluorescence.

Paper Details

Date Published: 21 April 2016
PDF: 7 pages
Proc. SPIE 9917, Saratov Fall Meeting 2015: Third International Symposium on Optics and Biophotonics and Seventh Finnish-Russian Photonics and Laser Symposium (PALS), 991713 (21 April 2016); doi: 10.1117/12.2229850
Show Author Affiliations
Nadezda Zhdanova, M.V. Lomonosov Moscow State Univ. (Russian Federation)
Evgeny Shirshin, M.V. Lomonosov Moscow State Univ. (Russian Federation)
Victor Fadeev, M.V. Lomonosov Moscow State Univ. (Russian Federation)
Alexander Priezzhev, M.V. Lomonosov Moscow State Univ. (Russian Federation)


Published in SPIE Proceedings Vol. 9917:
Saratov Fall Meeting 2015: Third International Symposium on Optics and Biophotonics and Seventh Finnish-Russian Photonics and Laser Symposium (PALS)
Elina A. Genina; Valery V. Tuchin; Vladimir L. Derbov; Dmitry E. Postnov; Igor V. Meglinski; Kirill V. Larin; Alexander Borisovich Pravdin, Editor(s)

© SPIE. Terms of Use
Back to Top