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Proceedings Paper

Quantification of two forms of green sulfur bacteria in their natural habitat using bacteriochlorophyll fluorescence spectra
Author(s): Anastasia V. Kharcheva; Anna A. Zhiltsova; Olga N. Lunina; Alexander S. Savvichev; Svetlana V. Patsaeva
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Paper Abstract

Detection of phototropic organisms in their natural habitat using optical instruments operating under water is urgently needed for many tasks of ecological monitoring. While fluorescence methods are widely applied nowadays to detect and characterize phytoplankton communities, the techniques for detection and recognition of anoxygenic phototrophs are considered challenging. Differentiation of the forms of anoxygenic green sulfur bacteria in natural water using spectral techniques remains problematic. Green sulfur bacteria could be found in two forms, green-colored (containing BChl d in pigment compound) and brown-colored (containing BChl e), have the special ecological niche in such reservoirs. Separate determination of these microorganisms by spectral methods is complicated because of similarity of spectral characteristics of their pigments. We describe the novel technique of quantification of two forms of green sulfur bacteria directly in water using bacteriochlorophyll fluorescence without pigment extraction. This technique is noninvasive and could be applied in remote mode in the water bodies with restricted water circulation to determine simultaneously concentrations of two forms of green sulfur bacteria in their natural habitat.

Paper Details

Date Published: 21 April 2016
PDF: 8 pages
Proc. SPIE 9917, Saratov Fall Meeting 2015: Third International Symposium on Optics and Biophotonics and Seventh Finnish-Russian Photonics and Laser Symposium (PALS), 99170P (21 April 2016); doi: 10.1117/12.2229848
Show Author Affiliations
Anastasia V. Kharcheva, Lomonosov Moscow State Univ. (Russian Federation)
Anna A. Zhiltsova, Lomonosov Moscow State Univ. (Russian Federation)
Olga N. Lunina, Winogradsky Institute of Microbiology (Russian Federation)
Alexander S. Savvichev, Winogradsky Institute of Microbiology (Russian Federation)
Svetlana V. Patsaeva, Lomonosov Moscow State Univ. (Russian Federation)


Published in SPIE Proceedings Vol. 9917:
Saratov Fall Meeting 2015: Third International Symposium on Optics and Biophotonics and Seventh Finnish-Russian Photonics and Laser Symposium (PALS)
Elina A. Genina; Valery V. Tuchin; Vladimir L. Derbov; Dmitry E. Postnov; Igor V. Meglinski; Kirill V. Larin; Alexander Borisovich Pravdin, Editor(s)

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