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Proceedings Paper

The development of attenuation compensation models of fluorescence spectroscopy signals
Author(s): Victor V. Dremin; Evgeny A. Zherebtsov; Ilya E. Rafailov; Andrey Y. Vinokurov; Irina N. Novikova; Angelina I. Zherebtsova; Karina S. Litvinova; Andrey V Dunaev
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Paper Abstract

This study examines the effect of blood absorption on the endogenous fluorescence signal intensity of biological tissues. Experimental studies were conducted to identify these effects. To register the fluorescence intensity, the fluorescence spectroscopy method was employed. The intensity of the blood flow was measured by laser Doppler flowmetry.

We proposed one possible implementation of the Monte Carlo method for the theoretical analysis of the effect of blood on the fluorescence signals. The simulation is constructed as a four-layer skin optical model based on the known optical parameters of the skin with different levels of blood supply. With the help of the simulation, we demonstrate how the level of blood supply can affect the appearance of the fluorescence spectra.

In addition, to describe the properties of biological tissue, which may affect the fluorescence spectra, we turned to the method of diffuse reflectance spectroscopy (DRS). Using the spectral data provided by the DRS, the tissue attenuation effect can be extracted and used to correct the fluorescence spectra.

Paper Details

Date Published: 21 April 2016
PDF: 6 pages
Proc. SPIE 9917, Saratov Fall Meeting 2015: Third International Symposium on Optics and Biophotonics and Seventh Finnish-Russian Photonics and Laser Symposium (PALS), 99170Y (21 April 2016); doi: 10.1117/12.2229451
Show Author Affiliations
Victor V. Dremin, State Univ. Education-Science-Production Complex (Russian Federation)
Evgeny A. Zherebtsov, State Univ. Education-Science-Production Complex (Russian Federation)
Ilya E. Rafailov, Aston Univ. (United Kingdom)
Andrey Y. Vinokurov, State Univ. Education-Science-Production Complex (Russian Federation)
Irina N. Novikova, State Univ. Education-Science-Production Complex (Russian Federation)
Angelina I. Zherebtsova, State Univ. Education-Science-Production Complex (Russian Federation)
Karina S. Litvinova, Aston Univ. (United Kingdom)
Andrey V Dunaev, State Univ. Education-Science-Production Complex (Russian Federation)


Published in SPIE Proceedings Vol. 9917:
Saratov Fall Meeting 2015: Third International Symposium on Optics and Biophotonics and Seventh Finnish-Russian Photonics and Laser Symposium (PALS)
Elina A. Genina; Valery V. Tuchin; Vladimir L. Derbov; Dmitry E. Postnov; Igor V. Meglinski; Kirill V. Larin; Alexander Borisovich Pravdin, Editor(s)

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