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Proceedings Paper

Wide-field TCSPC-based fluorescence lifetime imaging (FLIM) microscopy
Author(s): Klaus Suhling; Liisa M. Hirvonen; Wolfgang Becker; Stefan Smietana; Holger Netz; James Milnes; Thomas Conneely; Alix Le Marois; Ottmar Jagutzki
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Paper Abstract

Time-correlated single photon counting (TCSPC) is a widely used, sensitive, precise, robust and mature technique to measure photon arrival times in applications such as fluorescence spectroscopy and microscopy, light detection and ranging (lidar) and optical tomography. Wide-field TCSPC detection techniques, where the position and the arrival time of the photons are recorded simultaneously, have seen several advances in the last few years, from the microsecond to the picosecond time scale. Here, we summarise some of our recent work in this field with emphasis on microsecond resolution phosphorescence lifetime imaging (PLIM) and nanosecond fluorescence lifetime imaging (FLIM) microscopy.

Paper Details

Date Published: 5 May 2016
PDF: 10 pages
Proc. SPIE 9858, Advanced Photon Counting Techniques X, 98580J (5 May 2016); doi: 10.1117/12.2227198
Show Author Affiliations
Klaus Suhling, King's College London (United Kingdom)
Liisa M. Hirvonen, King's College London (United Kingdom)
Wolfgang Becker, Becker & Hickl GmbH (Germany)
Stefan Smietana, Becker & Hickl GmbH (Germany)
Holger Netz, Becker & Hickl GmbH (Germany)
James Milnes, Photek Ltd. (United Kingdom)
Thomas Conneely, Photek Ltd. (United Kingdom)
Alix Le Marois, King's College London (United Kingdom)
Ottmar Jagutzki, Institut für Kernphysik (Germany)


Published in SPIE Proceedings Vol. 9858:
Advanced Photon Counting Techniques X
Mark A. Itzler; Joe C. Campbell, Editor(s)

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