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Proceedings Paper

Hybrid nanosensor for colorimetric and ultrasensitive detection of nuclease contaminations
Author(s): Paola Cecere; Paola Valentini; Pier Paolo Pompa
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Paper Abstract

Nucleases are ubiquitous enzymes that degrade DNA or RNA, thus they can prejudice the good outcome of molecular biology experiments involving nucleic acids. We propose a colorimetric test for the naked-eye detection of nuclease contaminations. The system uses an hybrid nanosensor, based on gold nanoparticles functionalized with DNA probes. Our assay is rapid, instrument-free, simple and low-cost. Moreover, it reaches sensitivity equal or better than those of commercial kits, and presents a lot of advantageous aspects. Therefore, it is very competitive, with a real market potential. This test will be relevant in routine process monitoring in scientific laboratories, and in quality control in clinical laboratories and industrial processes, allowing the simultaneous detection of nucleases with different substrate specificities and large-scale screening.

Paper Details

Date Published: 27 April 2016
PDF: 7 pages
Proc. SPIE 9887, Biophotonics: Photonic Solutions for Better Health Care V, 98870O (27 April 2016); doi: 10.1117/12.2225929
Show Author Affiliations
Paola Cecere, Istituto Italiano di Tecnologia (Italy)
Univ. of Salento (Italy)
Paola Valentini, Istituto Italiano di Tecnologia (Italy)
Pier Paolo Pompa, Istituto Italiano di Tecnologia (Italy)

Published in SPIE Proceedings Vol. 9887:
Biophotonics: Photonic Solutions for Better Health Care V
Jürgen Popp; Valery V. Tuchin; Dennis L. Matthews; Francesco Saverio Pavone, Editor(s)

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