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Proceedings Paper

Photothermal damage is correlated to the delivery rate of time-integrated temperature
Author(s): Michael L. Denton; Gary D. Noojin; B. Giovanna Gamboa; Elharith M. Ahmed; Benjamin A. Rockwell
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Paper Abstract

Photothermal damage rate processes in biological tissues are usually characterized by a kinetics approach. This stems from experimental data that show how the transformation of a specified biological property of cells or biomolecule (plating efficiency for viability, change in birefringence, tensile strength, etc.) is dependent upon both time and temperature. However, kinetic methods require determination of kinetic rate constants and knowledge of substrate or product concentrations during the reaction. To better understand photothermal damage processes we have identified temperature histories of cultured retinal cells receiving minimum lethal thermal doses for a variety of laser and culture parameters. These “threshold” temperature histories are of interest because they inherently contain information regarding the fundamental thermal dose requirements for damage in individual cells. We introduce the notion of time-integrated temperature (Tint) as an accumulated thermal dose (ATD) with units of °C s. Damaging photothermal exposure raises the rate of ATD accumulation from that of the ambient (e.g. 37 °C) to one that correlates with cell death (e.g. 52 °C). The degree of rapid increase in ATD (ΔATD) during photothermal exposure depends strongly on the laser exposure duration and the ambient temperature.

Paper Details

Date Published: 7 March 2016
PDF: 11 pages
Proc. SPIE 9706, Optical Interactions with Tissue and Cells XXVII, 97061M (7 March 2016); doi: 10.1117/12.2225024
Show Author Affiliations
Michael L. Denton, Engility Corp. (United States)
Gary D. Noojin, Engility Corp. (United States)
B. Giovanna Gamboa, Engility Corp. (United States)
Elharith M. Ahmed, Engility Corp. (United States)
Benjamin A. Rockwell, Air Force Research Lab. (United States)


Published in SPIE Proceedings Vol. 9706:
Optical Interactions with Tissue and Cells XXVII
E. Duco Jansen, Editor(s)

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