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Proceedings Paper

Handheld chem/biosensor using extreme conformational changes in designed binding proteins to enhance surface plasmon resonance (SPR)
Author(s): Lori A. Lepak; Peter Schnatz; Igor Bendoym; Derek Kosciolek; Ronald Koder; David T. Crouse
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Paper Abstract

We present research results centered on development of a highly sensitive handheld chem/biosensor device using a novel class of engineered proteins, designed to undergo extreme conformational changes upon binding their target, which in turn cause extreme changes in refractive index in the protein layer. These proteins are attached to a detector chip with a structured metasurface, to translate the refractive index change into an enhanced shift in surface plasmon resonances (SPR), thereby improving the sensitivity of the overall detector relatively to current commercially available SPR systems. Theoretical calculations have demonstrated the potential of the conformational changes in the engineered proteins to provide the desired change in refractive index. A plasmonic chip with a simple grating metasurface structure was designed to maximize the SPR shift. A prototype chip and a prototype for the overall device housing were fabricated with the inclusion of all other required (commercially available) optical components. The proposed device holds considerable promise as a low-cost, highly sensitive, field-deployable detection system for chemical and biological toxins.

Paper Details

Date Published: 9 May 2016
PDF: 12 pages
Proc. SPIE 9862, Advanced Environmental, Chemical, and Biological Sensing Technologies XIII, 986208 (9 May 2016); doi: 10.1117/12.2222305
Show Author Affiliations
Lori A. Lepak, Phoebus Optoelectronics, LLC (United States)
Peter Schnatz, The City College of New York (United States)
Igor Bendoym, Phoebus Optoelectronics, LLC (United States)
Derek Kosciolek, The City College of New York (United States)
Ronald Koder, Phoebus Optoelectronics, LLC (United States)
The City College of New York (United States)
David T. Crouse, Phoebus Optoelectronics, LLC (United States)
Clarkson Univ. (United States)


Published in SPIE Proceedings Vol. 9862:
Advanced Environmental, Chemical, and Biological Sensing Technologies XIII
Tuan Vo-Dinh; Robert A. Lieberman; Günter G. Gauglitz, Editor(s)

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