Share Email Print
cover

Proceedings Paper

Multi-pulse pumping for far-field super-resolution imaging
Author(s): Sebastian Requena; Sangram Raut; Hung Doan; Joe Kimball; Rafal Fudala; Julian Borejdo; Ignacy Gryczynski; Yuri Strzhemechny; Zygmunt Gryczynski
Format Member Price Non-Member Price
PDF $14.40 $18.00

Paper Abstract

Recently, far-field optical imaging with a resolution significantly beyond diffraction limit has attracted tremendous attention allowing for high resolution imaging in living objects. Various methods have been proposed that are divided in to two basic approaches; deterministic super-resolution like STED or RESOLFT and stochastic super-resolution like PALM or STORM. We propose to achieve super-resolution in far-field fluorescence imaging by the use of controllable (on-demand) bursts of pulses that can change the fluorescence signal of long-lived component over one order of magnitude. We demonstrate that two beads, one labeled with a long-lived dye and another with a short-lived dye, separated by a distance lower than 100 nm can be easily resolved in a single experiment. The proposed method can be used to separate two biological structures in a cell by targeting them with two antibodies labeled with long-lived and short-lived fluorophores.

Paper Details

Date Published: 1 March 2016
PDF: 6 pages
Proc. SPIE 9714, Single Molecule Spectroscopy and Superresolution Imaging IX, 97140F (1 March 2016); doi: 10.1117/12.2221921
Show Author Affiliations
Sebastian Requena, Texas Christian Univ. (United States)
Sangram Raut, Texas Christian Univ. (United States)
Univ. of North Texas Health Science Ctr. (United States)
Hung Doan, Texas Christian Univ. (United States)
Joe Kimball, Texas Christian Univ. (United States)
Rafal Fudala, Univ. of North Texas Health Science Ctr. (United States)
Julian Borejdo, Univ. of North Texas Health Science Ctr. (United States)
Ignacy Gryczynski, Univ. of North Texas Health Science Ctr. (United States)
Yuri Strzhemechny, Texas Christian Univ. (United States)
Zygmunt Gryczynski, Texas Christian Univ. (United States)
Univ. of North Texas Health Science Ctr. (United States)


Published in SPIE Proceedings Vol. 9714:
Single Molecule Spectroscopy and Superresolution Imaging IX
Jörg Enderlein; Ingo Gregor; Zygmunt Karol Gryczynski; Rainer Erdmann; Felix Koberling, Editor(s)

© SPIE. Terms of Use
Back to Top