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Proceedings Paper

Investigation of prostate cancer cells using NADH and Tryptophan as biomarker: multiphoton FLIM-FRET microscopy
Author(s): Shagufta Rehman; Meghan J. O'Melia; Horst Wallrabe; Zdenek Svindrych; Dhyan Chandra; Ammasi Periasamy
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Paper Abstract

Fluorescence Lifetime Imaging (FLIM) can be used to understand the metabolic activity in cancer. Prostate cancer is one of the leading cancers in men in the USA. This research focuses on FLIM measurements of NAD(P)H and Tryptophan, used as biomarkers to understand the metabolic activity in prostate cancer cells. Two prostate cancers and one normal cell line were used for live-cell FLIM measurements on Zeiss780 2P confocal microscope with SPCM FLIM board. Glucose uptake and glycolysis proceeds about ten times faster in cancer than in non-cancerous tissues. Therefore, we assessed the glycolytic activity in the prostate cancer in comparison to the normal cells upon glucose stimulation by analyzing the NAD(P)H and Trp lifetime distribution and efficiency of energy transfer (E%). Furthermore, we treated the prostate cancer cells with 1μM Doxorubicin, a commonly used anti-cancer chemotherapeutic. Increase in NADH a2%, an indicator of increased glycolysis and increased E% between Trp and NAD(P)H were seen upon glucose stimulation for 30min. The magnitude of shift to the right for NAD(P)H a2% and E% distribution was higher in prostate cancer versus the normal cells. Upon treatment with Doxorubicin decrease in cellular metabolism was seen at 15 and 30 minutes. The histogram for NAD(P)H a2% post-treatment for prostate cancer cells showed a left shift compared to the untreated control suggesting decrease in glycolysis and metabolic activity opposite to what was observed after glucose stimulation. Hence, NAD(P)H and Trp lifetimes can be used biomarkers to understand metabolic activity in prostate cancer and upon chemotherapeutic interventions.

Paper Details

Date Published: 14 March 2016
PDF: 5 pages
Proc. SPIE 9712, Multiphoton Microscopy in the Biomedical Sciences XVI, 97120Q (14 March 2016); doi: 10.1117/12.2220779
Show Author Affiliations
Shagufta Rehman, The W.M. Keck Ctr. for Cellular Imaging, Univ. of Virginia (United States)
Meghan J. O'Melia, The W.M. Keck Ctr. for Cellular Imaging, Univ. of Virginia (United States)
Horst Wallrabe, The W.M. Keck Ctr. for Cellular Imaging, Univ. of Virginia (United States)
Zdenek Svindrych, The W.M. Keck Ctr. for Cellular Imaging, Univ. of Virginia (United States)
Dhyan Chandra, Roswell Park Cancer Institute (United States)
Ammasi Periasamy, The W.M. Keck Ctr. for Cellular Imaging, Univ. of Virginia (United States)


Published in SPIE Proceedings Vol. 9712:
Multiphoton Microscopy in the Biomedical Sciences XVI
Ammasi Periasamy; Peter T. C. So; Karsten König, Editor(s)

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