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Proceedings Paper

Fiber optic biofluorometer for physiological research on muscle slices
Author(s): Mathias Belz; Andreas Dendorfer; Jan Werner; Daniel Lambertz; Karl-Friedrich Klein
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Paper Abstract

A focus of research in cell physiology is the detection of Ca2+, NADH, FAD, ATPase activity or membrane potential, only to name a few, in muscle tissues. In this work, we report on a biofluorometer using ultraviolet light emitting diodes (UV-LEDs), optical fibers and two photomultipliers (PMTs) using synchronized fluorescence detection with integrated background correction to detect free calcium, Ca2+, in cardiac muscle tissue placed in a horizontal tissue bath and a microscope setup. Fiber optic probes with imaging optics have been designed to transport excitation light from the biofluorometer's light output to a horizontal tissue bath and to collect emission light from a tissue sample of interest to two PMTs allowing either single excitation / single emission or ratiometric, dual excitation / single emission or single excitation / dual emission fluorescence detection of indicator dyes or natural fluorophores. The efficient transport of light from the excitation LEDs to the tissue sample, bleaching effects of the excitation light in both, polymer and fused silica-based fibers will be discussed. Furthermore, a new approach to maximize light collection of the emission light using high NA fibers and high NA coupling optics will be shown. Finally, first results on Ca2+ measurements in cardiac muscle slices in a traditional microscope setup and a horizontal tissue bath using fiber optic probes will be introduced and discussed.

Paper Details

Date Published: 17 March 2016
PDF: 11 pages
Proc. SPIE 9702, Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications XVI, 97020Q (17 March 2016); doi: 10.1117/12.2220291
Show Author Affiliations
Mathias Belz, World Precision Instruments (Germany)
Andreas Dendorfer, Ludwig-Maximilians-Univ. (Germany)
Jan Werner, Technische Hochschule Mittelhessen (Germany)
Daniel Lambertz, World Precision Instruments Germany GmbH (Germany)
Karl-Friedrich Klein, Technische Hochschule Mittelhessen (Germany)

Published in SPIE Proceedings Vol. 9702:
Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications XVI
Israel Gannot, Editor(s)

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