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Proceedings Paper

High resolution microendoscopy with structured illumination and Lugol's iodine staining for evaluation of breast cancer architecture
Author(s): Jessica Dobbs; Matthew Kyrish; Savitri Krishnamurthy; Benjamin Grant; Henry Kuerer; Wei Yang; Tomasz Tkaczyk; Rebecca Richards-Kortum
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Paper Abstract

Intraoperative margin assessment to evaluate resected tissue margins for neoplastic tissue is performed to prevent reoperations following breast-conserving surgery. High resolution microendoscopy (HRME) can rapidly acquire images of fresh tissue specimens, but is limited by low image contrast in tissues with high optical scattering. In this study we evaluated two techniques to reduce out-of-focus light: HRME image acquisition with structured illumination (SI-HRME) and topical application of Lugol’s Iodine. Fresh breast tissue specimens from 19 patients were stained with proflavine alone or Lugol’s Iodine and proflavine. Images of tissue specimens were acquired using a confocal microscope and an HRME system with and without structured illumination. Images were evaluated based on visual and quantitative assessment of image contrast. The highest mean contrast was measured in confocal images stained with proflavine. Contrast was significantly lower in HRME images stained with proflavine; however, incorporation of structured illumination significantly increased contrast in HRME images to levels comparable to that in confocal images. The addition of Lugol’s Iodine did not increase mean contrast significantly for HRME or SI-HRME images. These findings suggest that structured illumination could potentially be used to increase contrast in HRME images of breast tissue for rapid image acquisition.

Paper Details

Date Published: 7 March 2016
PDF: 11 pages
Proc. SPIE 9703, Optical Biopsy XIV: Toward Real-Time Spectroscopic Imaging and Diagnosis, 97030E (7 March 2016); doi: 10.1117/12.2219352
Show Author Affiliations
Jessica Dobbs, Rice Univ. (United States)
Matthew Kyrish, Rice Univ. (United States)
Savitri Krishnamurthy, The Univ. of Texas M.D. Anderson Cancer Ctr. (United States)
Benjamin Grant, Rice Univ. (United States)
Henry Kuerer, The Univ. of Texas M.D. Anderson Cancer Ctr. (United States)
Wei Yang, The Univ. of Texas M.D. Anderson Cancer Ctr. (United States)
Tomasz Tkaczyk, Rice Univ. (United States)
Rebecca Richards-Kortum, Rice Univ. (United States)

Published in SPIE Proceedings Vol. 9703:
Optical Biopsy XIV: Toward Real-Time Spectroscopic Imaging and Diagnosis
Robert R. Alfano; Stavros G. Demos, Editor(s)

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