Share Email Print
cover

Proceedings Paper

Label-free hyperspectral microscopy for scatter imaging of biological processes in cells (Conference Presentation)
Author(s): Jeeseong C. Hwang; Aniruddha Ray; Philip P. Cheney; Bonghwan Chon; Ji Youn Lee; Kimberly A. Briggman

Paper Abstract

We will present unique applications of a label-free, hyperspectral scatter imaging technique in different microscopy platforms including conventional wide-field, dark-field, and confocal. In different platforms, we conducted label-free imaging of cells undergoing biological processes such as nanoparticle uptake, apoptosis, and metabolic flux change in response to the variation of the osmotic pressure. Hyperspectral image analyses resolved spectral endmembers corresponding to unique scattering and absorption characteristics as a result of such processes at the single particle, single organelle, and single cell level, delineating the details of nanomaterial-cell interactions in a 2D cell culture, cell apoptotic characteristics in a 3D culture, and volumetric changes of single cells under the variation of osmotic pressure. Our label-free scatter imaging has the potential for a broad range of biological and biomedical applications such as the development of scatter-based imaging contrast agents and the measurement of scatter parameters of subcellular organelles to identify the sub-micron scale origins of scattering signals in tissue scattering measurements.

Paper Details

Date Published: 26 April 2016
PDF: 1 pages
Proc. SPIE 9700, Design and Quality for Biomedical Technologies IX, 97000L (26 April 2016); doi: 10.1117/12.2218639
Show Author Affiliations
Jeeseong C. Hwang, National Institute of Standards and Technology (United States)
Aniruddha Ray, National Institute of Standards and Technology (United States)
Philip P. Cheney, National Institute of Standards and Technology (United States)
Bonghwan Chon, National Institute of Standards and Technology (United States)
Ji Youn Lee, National Institute of Standards and Technology (United States)
Kimberly A. Briggman, National Institute of Standards and Technology (United States)


Published in SPIE Proceedings Vol. 9700:
Design and Quality for Biomedical Technologies IX
Ramesh Raghavachari; Rongguang Liang, Editor(s)

© SPIE. Terms of Use
Back to Top