Share Email Print
cover

Proceedings Paper

Universal lab-on-a-chip platform for complex, perfused 3D cell cultures
Author(s): F. Sonntag; F. Schmieder; J. Ströbel; S. Grünzner; M. Busek; K. Günther; T. Steege; C. Polk; U. Klotzbach
Format Member Price Non-Member Price
PDF $14.40 $18.00
cover GOOD NEWS! Your organization subscribes to the SPIE Digital Library. You may be able to download this paper for free. Check Access

Paper Abstract

The miniaturization, rapid prototyping and automation of lab-on-a-chip technology play nowadays a very important role. Lab-on-a-chip technology is successfully implemented not only for environmental analysis and medical diagnostics, but also as replacement of animals used for the testing of substances in the pharmaceutical and cosmetics industries. For that purpose the Fraunhofer IWS and partners developed a lab-on-a-chip platform for perfused cell-based assays in the last years, which includes different micropumps, valves, channels, reservoirs and customized cell culture modules. This technology is already implemented for the characterization of different human cell cultures and organoids, like skin, liver, endothelium, hair follicle and nephron. The advanced universal lab-on-a-chip platform for complex, perfused 3D cell cultures is divided into a multilayer basic chip with integrated micropump and application-specific 3D printed cell culture modules. Moreover a technology for surface modification of the printed cell culture modules by laser micro structuring and a complex and flexibly programmable controlling device based on an embedded Linux system was developed. A universal lab-on-a-chip platform with an optional oxygenator and a cell culture module for cubic scaffolds as well as first cell culture experiments within the cell culture device will be presented. The module is designed for direct interaction with robotic dispenser systems. This offers the opportunity to combine direct organ printing of cells and scaffolds with the microfluidic cell culture module. The characterization of the developed system was done by means of Micro-Particle Image Velocimetry (μPIV) and an optical oxygen measuring system.

Paper Details

Date Published: 21 March 2016
PDF: 12 pages
Proc. SPIE 9705, Microfluidics, BioMEMS, and Medical Microsystems XIV, 970516 (21 March 2016); doi: 10.1117/12.2218606
Show Author Affiliations
F. Sonntag, Fraunhofer Institute for Material and Beam Technology IWS (Germany)
F. Schmieder, Fraunhofer Institute for Material and Beam Technology IWS (Germany)
TU Dresden (Germany)
J. Ströbel, Fraunhofer Institute for Material and Beam Technology IWS (Germany)
S. Grünzner, Fraunhofer Institute for Material and Beam Technology IWS (Germany)
TU Dresden (Germany)
M. Busek, Fraunhofer Institute for Material and Beam Technology IWS (Germany)
K. Günther, TU Dresden (Germany)
T. Steege, Fraunhofer Institute for Material and Beam Technology IWS (Germany)
C. Polk, Fraunhofer Institute for Material and Beam Technology IWS (Germany)
U. Klotzbach, Fraunhofer Institute for Material and Beam Technology IWS (Germany)


Published in SPIE Proceedings Vol. 9705:
Microfluidics, BioMEMS, and Medical Microsystems XIV
Bonnie L. Gray; Holger Becker, Editor(s)

© SPIE. Terms of Use
Back to Top