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Proceedings Paper

Adaptive optics without guide stars (Conference Presentation)
Author(s): Jerome Mertz; Jiang Li; Devin Beaulieu; Hari P. Paudel; Roman Barankov; Thomas G. Bifano
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Paper Abstract

Adaptive optics is a strategy to compensate for sample-induced aberrations in microscopy applications. Generally, it requires the presence of "guide stars" in the sample to serve as localized reference targets. We describe an implementation of conjugate adaptive optics that is amenable to widefield (i.e. non-scanning) microscopy, and can provide aberration corrections over potentially large fields of view without the use of guide stars. A unique feature of our implementation is that it is based on wavefront sensing with a single-shot partitioned-aperture sensor that provides large dynamic range compatible with extended samples. Combined information provided by this sensor and the imaging camera enable robust image de-blurring based on a rapid estimation of sample and aberrations obtained by closed-loop feedback. We present the theoretical principle of our technique and experimental demonstrations using both trans-illumination and fluorescence microscopes. Finally, we apply our technique to mouse brain imaging.

Paper Details

Date Published: 27 April 2016
PDF: 1 pages
Proc. SPIE 9717, Adaptive Optics and Wavefront Control for Biological Systems II, 97170F (27 April 2016); doi: 10.1117/12.2218574
Show Author Affiliations
Jerome Mertz, Boston Univ. (United States)
Jiang Li, Boston Univ. (United States)
Devin Beaulieu, Boston Univ. (United States)
Hari P. Paudel, Boston Univ. (United States)
Roman Barankov, Boston Univ. (United States)
Thomas G. Bifano, Boston Univ. (United States)

Published in SPIE Proceedings Vol. 9717:
Adaptive Optics and Wavefront Control for Biological Systems II
Thomas G. Bifano; Joel Kubby; Sylvain Gigan, Editor(s)

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