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Proceedings Paper

Photodynamic antimicrobial therapy to inhibit pseudomonas aeruginosa of corneal isolates (Conference Presentation)
Author(s): Heather A. Durkee; Nidhi Relhan; Alejandro Arboleda; Francisco Halili; Carolina De Freitas; Karam Alawa; Mariela C. Aguilar; Guillermo Amescua; Darlene Miller; Jean-Marie Parel

Paper Abstract

Keratitis associated with Pseudomonas aeruginosa is difficult to manage. Treatment includes antibiotic eye drops, however, some strains of Pseudomonas aeruginosa are resistant. Current research efforts are focused on finding alternative and adjunct therapies to treat multi-drug resistant bacteria. One promising alternate technique is photodynamic therapy (PDT). The purpose of this study was to evaluate the effect of riboflavin- and rose bengal-mediated PDT on Pseudomonas aeruginosa keratitis isolates in vitro. Two isolates (S+U- and S-U+) of Pseudomonas aeruginosa were derived from keratitis patients and exposed to five experimental groups: (1) Control (dark, UV-A irradiation, 525nm irradiation); (2) 0.1% riboflavin (dark, UV-A irradiation); and (3) 0.1% rose bengal, (4) 0.05% rose bengal and (5) 0.01% rose bengal (dark, 525nm irradiation). Three days after treatment, in dark conditions of all concentration of riboflavin and rose bengal showed no inhibition in both S+U- and S-U+ strains of Pseudomonas aeruginosa. In 0.1% and 0.05% rose bengal irradiated groups, for both S+U- and S-U+ strains, there was complete inhibition of bacterial growth in the central 50mm zone corresponding to the diameter of the green light source. These in vitro results suggest that rose bengal photodynamic therapy may be an effective adjunct treatment for Pseudomonas aeruginosa keratitis.

Paper Details

Date Published: 26 April 2016
PDF: 1 pages
Proc. SPIE 9693, Ophthalmic Technologies XXVI, 96931C (26 April 2016); doi: 10.1117/12.2218183
Show Author Affiliations
Heather A. Durkee, Bascom Palmer Eye Institute, Univ. of Miami (United States)
Nidhi Relhan, Bascom Palmer Eye Institute, Univ. of Miami (United States)
Alejandro Arboleda, Bascom Palmer Eye Institute (United States)
Francisco Halili, Bascom Palmer Eye Institute, Univ. of Miami (United States)
Carolina De Freitas, Bascom Palmer Eye Institute, Univ. of Miami (United States)
Karam Alawa, Bascom Palmer Eye Institute, Univ. of Miami (United States)
Mariela C. Aguilar, Bascom Palmer Eye Institute, Univ. of Miami (United States)
Guillermo Amescua, Bascom Palmer Eye Institute, Univ. of Miami (United States)
Darlene Miller, Bascom Palmer Eye Institute, Univ. of Miami (United States)
Jean-Marie Parel, Bascom Palmer Eye Institute, Univ. of Miami (United States)
Univ. of Liège (Belgium)


Published in SPIE Proceedings Vol. 9693:
Ophthalmic Technologies XXVI
Fabrice Manns; Per G. Söderberg; Arthur Ho, Editor(s)

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