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Proceedings Paper

Fluorescent silica nanoparticles containing covalently bound dyes for reporter, marker, and sensor applications
Author(s): Gabor Patonay; Maged Henary; Gala Chapman; Kyle Emer; Sidney Crow
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Paper Abstract

Silica nanoparticles have proven to be useful in many bioanalytical and medical applications and have been used in numerous applications during the last decade. Combining the properties of silica nanoparticles and fluorescent dyes that may be used as chemical probes or labels can be relatively easy by simply soaking porous silica nanoparticles in a solution of the dye of interest. Under proper conditions the entrapped dye can stay inside the silica nanoparticle for several hours resulting in a useful probe. In spite of the relative durability of these probes, leaching can still occur. A much better approach is to synthesize silica nanoparticles that have the fluorescent dye covalently attached to the backbone structure of the silica nanoparticle. This can be achieved by using appropriately modified tetraethyl orthosilicate (TEOS) analogues during the silica nanoparticle synthesis. The molar ratio of TEOS and modified TEOS will determine the fluorescent dye load in the silica nanoparticle. Dependent on the chemical stability of the reporting dye either reverse micellar (RM) or Stöber method can be used for silica nanoparticle synthesis. If dye stability allows RM procedure is preferred as it results in a much easier control of the silica nanoparticle reaction itself. Also controlling the size and uniformity of the silica nanoparticles are much easier using RM method. Dependent on the functional groups present in the reporting dye used in preparation of the modified TEOS, the silica nanoparticles can be utilized in many applications such as pH sensor, metal ion sensors, labels, etc. In addition surface activated silica nanoparticles with reactive moieties are also excellent reporters or they can be used as bright fluorescent labels. Many different fluorescent dyes can be used to synthesize silica nanoparticles including visible and NIR dyes. Several bioanalytical applications are discussed including studying amoeba phagocytosis.

Paper Details

Date Published: 22 April 2016
PDF: 9 pages
Proc. SPIE 9723, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications VIII, 97230Q (22 April 2016); doi: 10.1117/12.2217869
Show Author Affiliations
Gabor Patonay, Georgia State Univ. (United States)
Maged Henary, Georgia State Univ. (United States)
Gala Chapman, Georgia State Univ. (United States)
Kyle Emer, Georgia State Univ. (United States)
Sidney Crow, Georgia State Univ. (United States)

Published in SPIE Proceedings Vol. 9723:
Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications VIII
Samuel Achilefu; Ramesh Raghavachari, Editor(s)

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