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Proceedings Paper

3D printed miniaturized spectral system for tissue fluorescence lifetime measurements
Author(s): Luwei Zou; Mohamad Mahmoud; Mehdi Fahs; Rui Liu; Joe F. Lo
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Paper Abstract

Various types of collagens, e.g. type I and III, represent the main load-bearing components in biological tissues. Their composition changes during processes like wound healing and fibrosis. Collagens exhibit autofluorescence when excited by ultra-violet light, distinguishable by their unique fluorescent lifetimes across a range of emission wavelengths. Therefore, we designed a miniaturized spectral-lifetime detection system for collagens as a non-invasive probe for monitoring tissue in wound healing and scarring applications. A sine modulated LED illumination was applied to enable frequency domain (FD) fluorescence lifetime measurements under different wavelengths bands, separated via a series of longpass dichroics at 387nm, 409nm and 435nm. To achieve the minute scale of optomechanics, we employed a stereolithography based 3D printer with <50 μm resolution to create a custom designed optical mount in a hand-held form factor. We examined the characteristics of the 3D printed optical system with finite element modeling to simulate the effect of thermal (LED) and mechanical (handling) strain on the optical system. Using this device, the phase shift and demodulation of collagen types were measured, where the separate spectral bands enhanced the differentiation of their lifetimes.

Paper Details

Date Published: 6 April 2016
PDF: 14 pages
Proc. SPIE 9711, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IX, 97111S (6 April 2016); doi: 10.1117/12.2217696
Show Author Affiliations
Luwei Zou, Univ. of Michigan-Dearborn (United States)
Mohamad Mahmoud, Univ. of Michigan-Dearborn (United States)
Mehdi Fahs, Univ. of Michigan-Dearborn (United States)
Rui Liu, Univ. of Michigan-Dearborn (United States)
Joe F. Lo, Univ. of Michigan-Dearborn (United States)

Published in SPIE Proceedings Vol. 9711:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IX
Daniel L. Farkas; Dan V. Nicolau; Robert C. Leif, Editor(s)

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