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Proceedings Paper

3D tissue engineered micro-tumors for optical-based therapeutic screening platform
Author(s): Joseph L. Spano; Trevor J. Schmitt; Ryan C. Bailey; Timothy S. Hannon; Mohamed Elmajdob; Eric M. Mason; Guochang Ye; Soumen Das; Sudipta Seal; Michael B. Fenn
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Paper Abstract

Melanoma is an underserved area of cancer research, with little focus on studying the effects of tumor extracellular matrix (ECM) properties on melanoma tumor progression, metastasis, and treatment efficacy. We’ve developed a Raman spectral mapping-based in-vitro screening platform that allows for nondestructive in-situ, multi-time point assessment of a novel potential nanotherapeutic adjuvant, nanoceria (cerium oxide nanoparticles), for treating melanoma. We’ve focused primarily on understanding melanoma tumor ECM composition and how it influences cell morphology and ICC markers. Furthermore, we aim to correlate this with studies on nanotherapeutic efficacy to coincide with the goal of predicting and preventing metastasis based on ECM composition. We’ve compiled a Raman spectral database for substrates containing varying compositions of fibronectin, elastin, laminin, and collagens type I and IV. Furthermore, we’ve developed a machine learning-based semi-quantitative analysis platform utilizing dimensionality reduction with subsequent pixel classification and semi-quantitation of ECM composition using Direct Classical Least Squares for classification and estimation of the reorganization of these components by taking 2D maps using Raman spectroscopy. Gaining an understanding of how tissue properties influence ECM organization has laid the foundation for future work utilizing Raman spectroscopy to assess therapeutic efficacy and matrix reorganization imparted by nanoceria. Specifically, this will allow us to better understand the role of HIF1a in matrix reorganization of the tumor microenvironment. By studying the relationship between substrate modulus and nanoceria’s ability to inhibit an ECM that is conducive to tumor formation, we endeavor to show that nanoceria may prevent or even revert tumor conducive microenvironments.

Paper Details

Date Published: 17 March 2016
PDF: 11 pages
Proc. SPIE 9707, Dynamics and Fluctuations in Biomedical Photonics XIII, 97070N (17 March 2016); doi: 10.1117/12.2217678
Show Author Affiliations
Joseph L. Spano, Florida Institute of Technology (United States)
Trevor J. Schmitt, Florida Institute of Technology (United States)
Ryan C. Bailey, Florida Institute of Technology (United States)
Timothy S. Hannon, Florida Institute of Technology (United States)
Mohamed Elmajdob, Florida Institute of Technology (United States)
Eric M. Mason, Florida Institute of Technology (United States)
Guochang Ye, Florida Institute of Technology (United States)
Soumen Das, Univ. of Central Florida (United States)
Sudipta Seal, Univ. of Central Florida (United States)
Michael B. Fenn, Florida Institute of Technology (United States)


Published in SPIE Proceedings Vol. 9707:
Dynamics and Fluctuations in Biomedical Photonics XIII
Valery V. Tuchin; Kirill V. Larin; Martin J. Leahy; Ruikang K. Wang, Editor(s)

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