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Proceedings Paper

Phase correlation imaging (PCI) for cell dynamics investigation (Conference Presentation)
Author(s): Lihong Ma; Rajshekhar Gannavarpu; Ru Wang; Basanta Bhaduri; Shamira Sridharan; Mustafa A. Mir; Arindam Chakraborty; Supriya G. Prasanth; Larry Millet; Martha U. Gillette; Gabriel Popescu
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Paper Abstract

We present a new method, referred to as phase correlation imaging (PCI), to study cell dynamics and function through temporal phase correlation analysis. PCI offers label-free, high-performance, simple-design, as well as suitability for operation in a conventional microscopy setting. PCI works without the need for controlled or synchronized photoactivation and sophisticated acquisition schemes, and only involves taking a sequence of phase images. The PCI image incorporates information on the phase fluctuations induced by both Brownian motion and deterministic motion of intracellular transport across large scales. We employed spatial light interference microscopy (SLIM) recently developed in our laboratory to experimentally measure quantitative phase information which renders the thickness and refractive index of cellular components without adding contrast agents. The acquisition process is repeated to obtain time-lapse phase images. We calculate the correlation time at each pixel for acquired time-lapse phase images and obtain the correlation time map in space. By temporal correlation analysis, PCI reveals cell dynamics information, which is complementary to quantitative phase imaging itself.

Paper Details

Date Published: 27 April 2016
PDF: 1 pages
Proc. SPIE 9718, Quantitative Phase Imaging II, 97180P (27 April 2016); doi: 10.1117/12.2217666
Show Author Affiliations
Lihong Ma, Univ. of Illinois at Urbana-Champaign (United States)
Zhejiang Normal Univ. (China)
Rajshekhar Gannavarpu, Univ. of Illinois at Urbana-Champaign (United States)
Ru Wang, Univ. of Illinois at Urbana-Champaign (United States)
Basanta Bhaduri, Univ. of Illinois at Urbana-Champaign (United States)
Shamira Sridharan, Univ. of Illinois at Urbana-Champaign (United States)
Mustafa A. Mir, Univ. of Illinois at Urbana-Champaign (United States)
Arindam Chakraborty, Univ. of Illinois at Urbana-Champaign (United States)
Supriya G. Prasanth, Univ. of Illinois at Urbana-Champaign (United States)
Larry Millet, Oak Ridge National Lab. (United States)
Martha U. Gillette, Univ. of Illinois at Urbana-Champaign (United States)
Gabriel Popescu, Univ. of Illinois at Urbana-Champaign (United States)


Published in SPIE Proceedings Vol. 9718:
Quantitative Phase Imaging II
Gabriel Popescu; YongKeun Park, Editor(s)

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