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Proceedings Paper

Immobilization of antibodies onto gold and titanium oxide surfaces
Author(s): Marie-Claude Millot; Francoise Martin; Juliette Omont; Bernard Sebille; Yves Levy
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Paper Abstract

The immobilization of antibodies onto surfaces by means of a reactive polymer has been studied. Two types of surfaces, gold and titanium oxide, were investigated. After modification with a suitable reagent bearing amino group, these surfaces are able to link covalently the reactive polymer through its N-hydroxysuccinimide carbonate functions. After polymer immobilization, a reaction between antibody and the remaining reactive sites on the polymer can take palce. This permits a covalent binding of biomolecules to the surface. The resulting biosensor is stable and specific. Another approach has been used for immobilization of antibodies: it involves a modification of the polymer with sulfonate groups and fixation of tetravalent metal ions. The resulting biosensor can recognize specifically the corresponding antigen and the regeneration of the surface between polymer and antibody layers is possible.

Paper Details

Date Published: 29 September 1995
PDF: 8 pages
Proc. SPIE 2508, Chemical, Biochemical, and Environmental Fiber Sensors VII, (29 September 1995); doi: 10.1117/12.221728
Show Author Affiliations
Marie-Claude Millot, Univ. Paris XII (France)
Francoise Martin, Institut d'Optique (France)
Juliette Omont, Univ. Paris XII (France)
Bernard Sebille, Univ. Paris XII (France)
Yves Levy, Institut d'Optique (France)

Published in SPIE Proceedings Vol. 2508:
Chemical, Biochemical, and Environmental Fiber Sensors VII
Annamaria V. Scheggi, Editor(s)

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