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Proceedings Paper

Microscopic validation of whole mouse micro-metastatic tumor imaging agents using cryo-imaging and sliding organ image registration
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Paper Abstract

We created a metastasis imaging, analysis platform consisting of software and multi-spectral cryo-imaging system suitable for evaluating emerging imaging agents targeting micro-metastatic tumor. We analyzed CREKA-Gd in MRI, followed by cryo-imaging which repeatedly sectioned and tiled microscope images of the tissue block face, providing anatomical bright field and molecular fluorescence, enabling 3D microscopic imaging of the entire mouse with single metastatic cell sensitivity. To register MRI volumes to the cryo bright field reference, we used our standard mutual information, non-rigid registration which proceeded: preprocess → affine → B-spline non-rigid 3D registration. In this report, we created two modified approaches: mask where we registered locally over a smaller rectangular solid, and sliding organ. Briefly, in sliding organ, we segmented the organ, registered the organ and body volumes separately and combined results. Though sliding organ required manual annotation, it provided the best result as a standard to measure other registration methods. Regularization parameters for standard and mask methods were optimized in a grid search. Evaluations consisted of DICE, and visual scoring of a checkerboard display. Standard had accuracy of 2 voxels in all regions except near the kidney, where there were 5 voxels sliding. After mask and sliding organ correction, kidneys sliding were within 2 voxels, and Dice overlap increased 4%–10% in mask compared to standard. Mask generated comparable results with sliding organ and allowed a semi-automatic process.

Paper Details

Date Published: 29 March 2016
PDF: 10 pages
Proc. SPIE 9788, Medical Imaging 2016: Biomedical Applications in Molecular, Structural, and Functional Imaging, 97882L (29 March 2016); doi: 10.1117/12.2216981
Show Author Affiliations
Yiqiao Liu, Case Western Reserve Univ. (United States)
Bo Zhou, Case Western Reserve Univ. (United States)
Mohammed Qutaish, Case Western Reserve Univ. (United States)
David L. Wilson, Case Western Reserve Univ. (United States)

Published in SPIE Proceedings Vol. 9788:
Medical Imaging 2016: Biomedical Applications in Molecular, Structural, and Functional Imaging
Barjor Gimi; Andrzej Krol, Editor(s)

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