Share Email Print
cover

Proceedings Paper

Comparison of volume estimation methods for pancreatic islet cells
Author(s): Jiří Dvořák; Jan Švihlík; David Habart; Jan Kybic
Format Member Price Non-Member Price
PDF $14.40 $18.00

Paper Abstract

In this contribution we study different methods of automatic volume estimation for pancreatic islets which can be used in the quality control step prior to the islet transplantation. The total islet volume is an important criterion in the quality control. Also, the individual islet volume distribution is interesting — it has been indicated that smaller islets can be more effective. A 2D image of a microscopy slice containing the islets is acquired. The input of the volume estimation methods are segmented images of individual islets. The segmentation step is not discussed here. We consider simple methods of volume estimation assuming that the islets have spherical or ellipsoidal shape. We also consider a local stereological method, namely the nucleator. The nucleator does not rely on any shape assumptions and provides unbiased estimates if isotropic sections through the islets are observed. We present a simulation study comparing the performance of the volume estimation methods in different scenarios and an experimental study comparing the methods on a real dataset.

Paper Details

Date Published: 29 March 2016
PDF: 6 pages
Proc. SPIE 9788, Medical Imaging 2016: Biomedical Applications in Molecular, Structural, and Functional Imaging, 97881K (29 March 2016); doi: 10.1117/12.2216783
Show Author Affiliations
Jiří Dvořák, Charles Univ. in Prague (Czech Republic)
Czech Technical Univ. in Prague (Czech Republic)
Jan Švihlík, Univ. of Chemistry and Technology (Czech Republic)
Czech Technical Univ. in Prague (Czech Republic)
David Habart, Institute for Clinical and Experimental Medicine (Czech Republic)
Jan Kybic, Czech Technical Univ. in Prague (Czech Republic)


Published in SPIE Proceedings Vol. 9788:
Medical Imaging 2016: Biomedical Applications in Molecular, Structural, and Functional Imaging
Barjor Gimi; Andrzej Krol, Editor(s)

© SPIE. Terms of Use
Back to Top