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Proceedings Paper

Using quantitative interference phase microscopy for sperm acrosome evaluation (Conference Presentation)
Author(s): Michal Balberg; Ksawery Kalinowski; Mattan Levi; Natan T. Shaked
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Paper Abstract

We demonstrate quantitative assessment of sperm cell morphology, primarily acrosomal volume, using quantitative interference phase microscopy (IPM). Normally, the area of the acrosome is assessed using dyes that stain the acrosomal part of the cell. We have imaged fixed individual sperm cells using IPM. Following, the sample was stained and the same cells were imaged using bright field microscopy (BFM). We identified the acrosome using the stained BFM image, and used it to define a quantitative corresponding area in the IPM image and determine a quantitative threshold for evaluating the volume of the acrosome.

Paper Details

Date Published: 26 May 2016
PDF: 1 pages
Proc. SPIE 9718, Quantitative Phase Imaging II, 97180W (26 May 2016); doi: 10.1117/12.2216724
Show Author Affiliations
Michal Balberg, Tel Aviv Univ. (Israel)
Ksawery Kalinowski, Tel Aviv Univ. (Israel)
Mattan Levi, Tel Aviv Univ. (Israel)
Natan T. Shaked, Tel Aviv Univ. (Israel)


Published in SPIE Proceedings Vol. 9718:
Quantitative Phase Imaging II
Gabriel Popescu; YongKeun Park, Editor(s)

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