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Proceedings Paper

Label free detection of optogenetically stimulated cellular activity by low coherence interferometry (Conference Presentation)
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Paper Abstract

Detecting cellular activity in sub-millisecond timescale and micrometer resolution without using invasive means has been a long standing goal in the study of cellular networks. Here, we have employed phase sensitive low coherence interferometry for detecting optogenetically stimulated activity of cells. Nanoscale changes in optical path length (due to change in refractive index and changes in cell thickness) occur when cells are activated, which we aim to detect by phase sensitive low coherence interferometry. A low coherence interferometry and patch-clamp electrophysiology systems were integrated with an inverted fluorescence microscope. Blue laser beam was coupled to the electrophysiology-interferometric detection system for optogenetic stimulation. The phase-sensitive measurements were carried out on Channelrhodopsin-2 sensitized cells (identified by YFP fluorescence) as well as control cells in reflection mode for different intensities and exposures of optogenetic stimulation beam. This method offers good temporal and spatial resolution without using exogenous labeling. Results of studies on all optical stimulation and detection of cellular activity will be presented. Interpretation of the optical activity signals will be discussed in context with changes in cell physiology during stimulation. We will also discuss the potential sources of various artifacts in optical/electrical detection of cellular activity during optical stimulation.

Paper Details

Date Published: 26 April 2016
PDF: 1 pages
Proc. SPIE 9690, Clinical and Translational Neurophotonics; Neural Imaging and Sensing; and Optogenetics and Optical Manipulation, 96902D (26 April 2016); doi: 10.1117/12.2216404
Show Author Affiliations
Sarmishtha Satpathy, The Univ. of Texas at Arlington (United States)
Subrata Batabyal, The Univ. of Texas at Arlington (United States)
Digant P. Dave, The Univ. of Texas at Arlington (United States)
Samarendra K Mohanty, The Univ. of Texas at Arlington (United States)


Published in SPIE Proceedings Vol. 9690:
Clinical and Translational Neurophotonics; Neural Imaging and Sensing; and Optogenetics and Optical Manipulation
Steen J. Madsen; E. Duco Jansen; Samarendra K. Mohanty; Nitish V. Thakor; Qingming Luo; Victor X. D. Yang, Editor(s)

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