Share Email Print
cover

Proceedings Paper

Magnified image spatial spectrum (MISS) microscopy for high-speed QPI (Conference Presentation)
Author(s): Hassaan Majeed; Eun Jung Min; Sumin Kim; Woonggyu Jung; Catherine Best-Popescu; Gabriel Popescu
cover GOOD NEWS! Your organization subscribes to the SPIE Digital Library. You may be able to download this paper for free. Check Access

Paper Abstract

Diffraction Phase Microscopy (DPM) is a common-path, single shot QPI technique that has found applications in studies of red blood cell morphology and dynamics, cell growth measurement, as well as in Fourier Transform Light Scattering. In DPM, the phase is retrieved by interfering two orders of diffraction from a grating placed at the image plane. The reference field has been, in the past, generated by low pass filtering the zero order via a pinhole placed in the Fourier plane. For achieving the desired spatial coherence, the pinhole is often only 5-10 µm in diameter, making the system difficult to align every time an imaging study is performed. In this work, we eliminated the pinhole from the DPM optical path and generated instead the reference field by magnifying strongly the zero order. We show that a gradient-index (GRIN) lens (effective focal length of 300 µm) can be used to magnify the Fourier transform of the zero order to the point where the DC component fills the camera sensor. We show that the resulting Magnified Object Spectrum Interference Microscopy (MOSIM) system can successfully reconstruct quantitative phase images, without the need for tedious alignment. Because it conserves the common path geometry, MOSIM is characterized by 1.1 nm spatiotemporal pathlength noise. Since it is single shot, we demonstrated 400 frames/s acquisition. We anticipate that this new method can potentially lead to a more robust and less vibration sensitive QPI instrument for carrying out biological studies at various spatiotemporal scales.

Paper Details

Date Published: 27 April 2016
PDF: 1 pages
Proc. SPIE 9718, Quantitative Phase Imaging II, 97182T (27 April 2016); doi: 10.1117/12.2216130
Show Author Affiliations
Hassaan Majeed, Univ. of Illinois at Urbana-Champaign (United States)
Eun Jung Min, Univ. of Illinois at Urbana-Champaign (United States)
Sumin Kim, Univ. of Illinois at Urbana-Champaign (United States)
Woonggyu Jung, Ulsan National Institute of Science and Technology (Korea, Republic of)
Catherine Best-Popescu, Univ. of Illinois at Urbana-Champaign (United States)
Gabriel Popescu, Univ. of Illinois at Urbana-Champaign (United States)


Published in SPIE Proceedings Vol. 9718:
Quantitative Phase Imaging II
Gabriel Popescu; YongKeun Park, Editor(s)

© SPIE. Terms of Use
Back to Top