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Proceedings Paper

Rapid label-free chemical imaging of cells and tissues (Conference Presentation)

Paper Abstract

Coherent Raman imaging methods have been under development for almost 15 years. The field is beginning to mature, transitioning from a “new techniques” phase to an applications phase. I will discuss current capabilities of broadband coherent anti-Stokes Raman scattering (BCARS) microscopy using optimized excitation paradigms, and provide a few examples of how broadband BCARS imaging has helped to answer (or raise) questions in investigations of tissues and small organisms. I will also discuss progress in processing BCARS spectra to make them independent of excitation profile or non-resonant response, and directly comparable to spontaneous Raman spectra. I will also discuss progress on a new approach to time-domain BCARS that promises to significantly simplify and speed BCARS data acquisition.

Paper Details

Date Published: 27 April 2016
PDF: 1 pages
Proc. SPIE 9712, Multiphoton Microscopy in the Biomedical Sciences XVI, 97120C (27 April 2016); doi: 10.1117/12.2216118
Show Author Affiliations
Marcus T. Cicerone, National Institute of Standards and Technology (United States)
Charles H. Camp, National Institute of Standards and Technology (United States)


Published in SPIE Proceedings Vol. 9712:
Multiphoton Microscopy in the Biomedical Sciences XVI
Ammasi Periasamy; Peter T. C. So; Karsten König, Editor(s)

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