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Proceedings Paper

The role of DAMPS in ALA-PDT for skin squamous cell carcinoma (Conference Presentation)
Author(s): Xiuli Wang; Xiaojie Wang; Jie Ji; Haiyan Zhang; Lei Shi
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Paper Abstract

5-Aminolevulinic acid mediated photodynamic therapy (ALA-PDT) is an established local approach for skin squamous cell carcinoma. It is believed that dangerous signals damage-associated molecular patterns (DAMPs) play an important role in ALA-PDT. In this study, we evaluated in vitro and in vivo expressions of major DAMPs, calreticulin (CRT), heat shock proteins 70 (HSP70), and high mobility group box 1 (HMGB1), induced by ALA-PDT using immunohistochemistry, western blot, and ELISA in a squamous cell carcinoma (SCC) mouse model. The role of DAMPs in the maturation of DCs potentiated by ALA-PDT-treated tumor cells was detected by FACS and ELISA. Our results showed that ALA-PDT enhanced the expression of CRT, HSP70, and HMGB1. These induced DAMPs played an important role in activating DCs by PDT-treated tumor cells, including phenotypic maturation (upregulation of surface expression of MHC-II, CD80, and CD86) and functional maturation (enhanced capability to secrete IFN-γ and IL-12). Furthermore, injecting ALA-PDT-treated tumor cells into naïve mice resulted in complete protection against cancer cells of the same origin. Our findings indicate that ALA-PDT can upregulate DAMPs and enhance tumor immunogenicity, providing a promising strategy for inducing a systemic anticancer immune response.

Paper Details

Date Published: 27 April 2016
PDF: 1 pages
Proc. SPIE 9709, Biophotonics and Immune Responses XI, 970906 (27 April 2016); doi: 10.1117/12.2216078
Show Author Affiliations
Xiuli Wang, Shanghai Skin Disease Hospital (China)
Xiaojie Wang, Shanghai Skin Disease Hospital (China)
Jie Ji, Shanghai Skin Disease Hospital (China)
Haiyan Zhang, Shanghai Skin Disease Hospital (China)
Lei Shi, Shanghai Skin Disease Hospital (China)


Published in SPIE Proceedings Vol. 9709:
Biophotonics and Immune Responses XI
Wei R. Chen, Editor(s)

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