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Proceedings Paper

Conductivity affects nanosecond electrical pulse induced pressure transient formation
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Paper Abstract

Nanoporation occurs in cells exposed to high amplitude short duration (< 1μs) electrical pulses. The biophysical mechanism(s) responsible for nanoporation is unknown although several theories exist. Current theories focus exclusively on the electrical field, citing electrostriction, water dipole alignment and/or electrodeformation as the primary mechanisms for pore formation. Our group has shown that mechanical forces of substantial magnitude are also generated during nsEP exposures. We hypothesize that these mechanical forces may contribute to pore formation. In this paper, we report that alteration of the conductivity of the exposure solution also altered the level of mechanical forces generated during a nsEP exposure. By reducing the conductivity of the exposure solutions, we found that we could completely eliminate any pressure transients normally created by nsEP exposure. The data collected for this proceeding does not definitively show that the pressure transients previously identified contribute to nanoporation; however; it indicates that conductivity influences both survival and pressure transient formation.

Paper Details

Date Published: 7 March 2016
PDF: 8 pages
Proc. SPIE 9706, Optical Interactions with Tissue and Cells XXVII, 97060V (7 March 2016); doi: 10.1117/12.2214896
Show Author Affiliations
Caleb C. Roth, Univ. of Texas Health Science Ctr. San Antonio (United States)
Ronald A. Barnes, National Research Council (United States)
Bennett L. Ibey, Air Force Research Lab. (United States)
Hope T. Beier, Air Force Research Lab. (United States)
Randolph D. Glickman, Univ. of Texas Health Science Ctr. San Antonio (United States)


Published in SPIE Proceedings Vol. 9706:
Optical Interactions with Tissue and Cells XXVII
E. Duco Jansen, Editor(s)

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