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Proceedings Paper

Characterizations of individual human red blood cells from patients with diabetes mellitus (Conference Presentation)
Author(s): SangYun Lee; Seongsoo Jang; HyunJoo Park; YongKeun Park
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Paper Abstract

We systematically measure the morphological, biochemical, and biomechanical properties of individual human red blood cells (RBCs) from patients with diabetes mellitus using quantitative phase imaging technique to characterize the diabetic red cells with respect to those of the healthy. The 3-D refractive index tomograms and 2-D dynamic membrane fluctuation maps of individual RBCs are reconstructed from a set of the retrieved complex optical fields at various laser incidence angles using the Common-path diffraction optical tomography, from which volume, surface area, sphericity, hemoglobin (Hb) concentration, Hb content, and membrane fluctuation are obtained simultaneously. The correlative relations among the retrieved red cell indices of diabetic and healthy RBCs are also investigated with capabilities of individual cell measurement. As expected, there are no significant alterations in morphologies (cellular volumes, surface area, and sphericity) between diabetic and healthy RBCs. However, despite the minute mean corpuscular Hb differences in cell blood count datasheet, the measured Hb concentrations and Hb contents of diabetic RBCs are statistically higher than those of healthy RBCs, which might be related to the glycation of Hb molecules by hyperglycemia. Meanwhile, the membrane fluctuations of diabetic RBCs are clearly diminished compared to healthy red cells, implying the significantly decreased RBC deformability. In particular, it seems that the membrane fluctuations have mild negative relationships with the reported HbA1c levels.

Paper Details

Date Published: 27 April 2016
PDF: 1 pages
Proc. SPIE 9718, Quantitative Phase Imaging II, 97180X (27 April 2016); doi: 10.1117/12.2214861
Show Author Affiliations
SangYun Lee, KAIST (Korea, Republic of)
Seongsoo Jang, Univ. of Ulsan (Korea, Republic of)
Asan Medical Ctr. (Korea, Republic of)
HyunJoo Park, KAIST (Korea, Republic of)
YongKeun Park, KAIST (Korea, Republic of)


Published in SPIE Proceedings Vol. 9718:
Quantitative Phase Imaging II
Gabriel Popescu; YongKeun Park, Editor(s)

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