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Proceedings Paper

Monitoring femtosecond laser microscopic photothermolysis with multimodal microscopy (Conference Presentation)
Author(s): Yimei Huang; Harvey Lui; Jianhua Zhao; David I. McLean; Haishan Zeng
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Paper Abstract

Photothermolysis induced by femtosecond (fs) lasers may be a promising modality in dermatology because of its advantages of high precision due to multiphoton absorption and deeper penetration due to the use of near infrared wavelengths. Although multiphoton absorption nonlinear effects are capable of precision targeting, the femtosecond laser photothermolysis could still have effects beyond the targeted area if a sufficiently high dose of laser light is used. Such unintended effects could be minimized by real time monitoring photothermolysis during the treatment. Targeted photothermolytic treatment of ex vivo mouse skin dermis was performed with tightly focused fs laser beams. Images of reflectance confocal microscopy (RCM), second harmonic generation (SHG), and two-photon fluorescence (TPF) of the mouse skins were obtained with integrated multimodal microscopy before, during, and after the laser treatment. The RCM, SHG, and TPF signal intensities of the treatment areas changed after high power femtosecond laser irradiation. The intensities of the RCM and SHG signals decreased when the tissue was damaged, while the intensity of the TPF signal increased when the photothermolysis was achieved. Moreover, the TPF signal was more susceptible to the degree of the photothermolysis than the RCM and SHG signals. The results suggested that multimodal microscopy is a potentially useful tool to monitor and assess the femtosecond laser treatment of the skin to achieve microscopic photothermolysis with high precision.

Paper Details

Date Published: 27 April 2016
PDF: 1 pages
Proc. SPIE 9689, Photonic Therapeutics and Diagnostics XII, 96890P (27 April 2016); doi: 10.1117/12.2214798
Show Author Affiliations
Yimei Huang, The BC Cancer Agency Research Ctr. (Canada)
Harvey Lui, The Univ. of British Columbia (Canada)
Vancouver Coastal Health Research Institute (Canada)
Jianhua Zhao, The BC Cancer Agency Research Ctr. (Canada)
David I. McLean, The Univ. of British Columbia (Canada)
Vancouver Coastal Health Research Institute (Canada)
Haishan Zeng, The BC Cancer Agency Research Ctr. (Canada)

Published in SPIE Proceedings Vol. 9689:
Photonic Therapeutics and Diagnostics XII
Hyun Wook Kang; Guillermo J. Tearney; Melissa C. Skala; Bernard Choi; Andreas Mandelis; Brian J. F. Wong; Justus F. Ilgner; Nikiforos Kollias; Paul J. Campagnola; Kenton W. Gregory; Laura Marcu; Haishan Zeng, Editor(s)

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