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Proceedings Paper

Imaging of the stroke-related changes in the vascular system of the mouse brain with the use of extended focus optical coherence microscopy
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Paper Abstract

We used Optical Coherence Microscopy (OCM) to monitor structural and functional changes due to ischemic stroke in small animals brains in vivo. To obtain lateral resolution of 2.2 μm over the range of 600 μm we used extended focus configuration of OCM instrument involving Bessel beam. It provided access to detailed 3D information about the changes in brain vascular system up to the level of capillaries across I and II/III layers of neocortex. We used photothrombotic stroke model involving photoactive application of rose bengal to assure minimal invasiveness of the procedure and precise localization of the clot distribution center. We present the comparative analysis involving structural and angiographic maps of the stroke-affected brain enabling in-depth insight to the process of development of the disorder.

Paper Details

Date Published: 8 March 2016
PDF: 7 pages
Proc. SPIE 9697, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XX, 969718 (8 March 2016); doi: 10.1117/12.2214779
Show Author Affiliations
Szymon Tamborski, Nicolaus Copernicus Univ. (Poland)
Hong Chou Lyu, Nicolaus Copernicus Univ. (Poland)
Danuta Bukowska, Lion's Eye Institute (Australia)
Hubert Dolezyczek, Nencki Institute of Experimental Biology (Poland)
Grzegorz Wilczynski, Nencki Institute of Experimental Biology (Poland)
Daniel Szlag, Ecole Polytechnique Fédérale de Lausanne (Switzerland)
Theo Lasser, Ecole Polytechnique Fédérale de Lausanne (Switzerland)
Maciej Wojtkowski, Nicolaus Copernicus Univ. (Poland)
Maciej Szkulmowski, Nicolaus Copernicus Univ. (Poland)


Published in SPIE Proceedings Vol. 9697:
Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XX
Joseph A. Izatt; James G. Fujimoto; Valery V. Tuchin, Editor(s)

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