Share Email Print
cover

Proceedings Paper

Three dimensional time lapse imaging of live cell mitochondria with photothermal optical lock-in optical coherence microscopy (Conference Presentation)
Author(s): Miguel Sison; Sabyasachi Chakrabortty; Jerome Extermann; Amir Nahas; Christophe Pache; Tanja Weil; Theo Lasser
cover GOOD NEWS! Your organization subscribes to the SPIE Digital Library. You may be able to download this paper for free. Check Access

Paper Abstract

The photothermal optical lock-in optical coherence microscope (poli-OCM) introduced molecular specificity to OCM imaging, which is conventionally, a label-free technique. Here we achieve three-dimensional live cell and mitochondria specific imaging using ~4nm protein-functionalized gold nanoparticles (AuNPs). These nanoparticles do not photobleach and we demonstrate they’re suitability for long-term time lapse imaging. We compare the accuracy of labelling with these AuNPs using classical fluorescence confocal imaging with a standard mitochondria specific marker. Furthermore, time lapse poli-OCM imaging every 5 minutes over 1.5 hours period was achieved, revealing the ability for three-dimensional monitoring of mitochondria dynamics.

Paper Details

Date Published: 26 April 2016
PDF: 1 pages
Proc. SPIE 9697, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XX, 96971Y (26 April 2016); doi: 10.1117/12.2214761
Show Author Affiliations
Miguel Sison, Ecole Polytechnique Fédérale de Lausanne (Switzerland)
Sabyasachi Chakrabortty, Univ. Ulm (Germany)
Jerome Extermann, Ecole Polytechnique Fédérale de Lausanne (Switzerland)
Amir Nahas, Ecole Polytechnique Fédérale de Lausanne (Switzerland)
Christophe Pache, Ecole Polytechnique Fédérale de Lausanne (Switzerland)
Tanja Weil, Univ. Ulm (Germany)
Theo Lasser, Ecole Polytechnique Fédérale de Lausanne (Switzerland)


Published in SPIE Proceedings Vol. 9697:
Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XX
Joseph A. Izatt; James G. Fujimoto; Valery V. Tuchin, Editor(s)

© SPIE. Terms of Use
Back to Top