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Proceedings Paper

Structural and functional measurements of fertilized mouse oocytes with combined high-resolution OCT and inverted microscope (Conference Presentation)

Paper Abstract

We present a comprehensive imaging methodology for 3D structural and functional measurements of fertilized mouse oocytes. In contrary to methods used for mouse zygote imaging so far OCT provides 3D data without z axis movement of sample or objective lens. Furthermore, complex scanning protocols used in this study give access to different scales of repetition times and thus may become a tool for investigation of a different dynamic processes. Additionally, proposed scanning approach via variety of statistic operations can be used to enhance the quality of structural images. OCT system capabilities are presented and compared to standard microscopy. With a single 3D measurements one can extract 3D structure of the oocytes as well as en-face images that correspond to both bright and dark field microscopy. As an example of dynamic oocyte imaging pronuclei motion during development is presented. Limitations and possibilities of the new system are discussed.

Paper Details

Date Published: 26 April 2016
PDF: 1 pages
Proc. SPIE 9697, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XX, 96972B (26 April 2016); doi: 10.1117/12.2214736
Show Author Affiliations
Karol Karnowski, Nicolaus Copernicus Univ. (Poland)
Anna Ajduk, Univ. of Warsaw (Poland)
Maciej Wojtkowski, Nicolaus Copernicus Univ. (Poland)
Maciej Szkulmowski, Nicolaus Copernicus Univ. (Poland)


Published in SPIE Proceedings Vol. 9697:
Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XX
Joseph A. Izatt; James G. Fujimoto; Valery V. Tuchin, Editor(s)

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