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Proceedings Paper

Fully integrated reflection-mode photoacoustic/two-photon microscopy in vivo (Conference Presentation)
Author(s): Liang Song; Wei Song; Yang Zhang; Wei Zheng
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Paper Abstract

Using a water-immersion optical objective in conjunction with a miniature 40-MHz ultrasonic transducer, we developed reflection-mode photoacoustic microscopy with a transverse resolution as high as 320 nm. Here, we further integrated two-photon microscopy capability into the system to enable multimodality in vivo biomedical imaging at submicron resolution. As a result, the system is capable of tri-modality label-free imaging of microvasculature, collagen, and cell morphology, based on the contrast of optical absorption, second-harmonic generation, and autofluorescence, respectively. In addition, we demonstrated simultaneous microscopic imaging of neuron and microvasculature in the brain cortex of a living mouse, which may offer new opportunities for studying the mechanisms of neurovascular coupling.

Paper Details

Date Published: 27 April 2016
PDF: 1 pages
Proc. SPIE 9708, Photons Plus Ultrasound: Imaging and Sensing 2016, 97082H (27 April 2016); doi: 10.1117/12.2214685
Show Author Affiliations
Liang Song, Shenzhen Institute of Advanced Technology (China)
Wei Song, Shenzhen Institute of Advanced Technology (China)
Yang Zhang, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences (China)
Wei Zheng, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences (China)


Published in SPIE Proceedings Vol. 9708:
Photons Plus Ultrasound: Imaging and Sensing 2016
Alexander A. Oraevsky; Lihong V. Wang, Editor(s)

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