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Proceedings Paper

Ultrahigh resolution optical coherence elastography using a Bessel beam for extended depth of field
Author(s): Andrea Curatolo; Martin Villiger; Dirk Lorenser; Philip Wijesinghe; Alexander Fritz; Brendan F. Kennedy; David D. Sampson
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Paper Abstract

Visualizing stiffness within the local tissue environment at the cellular and sub-cellular level promises to provide insight into the genesis and progression of disease. In this paper, we propose ultrahigh-resolution optical coherence elastography, and demonstrate three-dimensional imaging of local axial strain of tissues undergoing compressive loading. The technique employs a dual-arm extended focus optical coherence microscope to measure tissue displacement under compression. The system uses a broad bandwidth supercontinuum source for ultrahigh axial resolution, Bessel beam illumination and Gaussian beam detection, maintaining sub-2 μm transverse resolution over nearly 100 μm depth of field, and spectral-domain detection allowing high displacement sensitivity. The system produces strain elastograms with a record resolution (x,y,z) of 2×2×15 μm. We benchmark the advances in terms of resolution and strain sensitivity by imaging a suitable inclusion phantom. We also demonstrate this performance on freshly excised mouse aorta and reveal the mechanical heterogeneity of vascular smooth muscle cells and elastin sheets, otherwise unresolved in a typical, lower resolution optical coherence elastography system.

Paper Details

Date Published: 8 March 2016
PDF: 8 pages
Proc. SPIE 9697, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XX, 96971Q (8 March 2016); doi: 10.1117/12.2214684
Show Author Affiliations
Andrea Curatolo, The Univ. of Western Australia (Australia)
Martin Villiger, Wellman Ctr. for Photomedicine, Massachusetts General Hospital (United States)
Harvard Medical School (United States)
Dirk Lorenser, The Univ. of Western Australia (Australia)
Philip Wijesinghe, The Univ. of Western Australia (Australia)
Alexander Fritz, The Univ. of Western Australia (Australia)
Brendan F. Kennedy, The Univ. of Western Australia (Australia)
David D. Sampson, The Univ. of Western Australia (Australia)

Published in SPIE Proceedings Vol. 9697:
Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XX
Joseph A. Izatt; James G. Fujimoto; Valery V. Tuchin, Editor(s)

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