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Proceedings Paper

Probing focal cortical dysplasia in formalin fixed samples using tissue optical spectroscopy
Author(s): Suresh Anand; Riccardo Cicchi; Flavio Giordano; Anna Maria Buccoliero; Valerio Conti; Renzo Guerrini; Francesco Saverio Pavone
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Paper Abstract

Focal cortical dysplasia (FCD) is one of most common causes of intractable epilepsy in pediatric population and these are often insensitive to anti-epileptic drugs. FCD is characterized by a disarray in localized regions of the cerebral cortex and abnormal neurons which results them to misfire with incorrect signals. Resective neurosurgery to remove or disconnect the affected parts from the rest of the brain seems to be a viable option to treat FCD. Before neurosurgery the subject could undergo imaging studies including magnetic resonance imaging (MRI) or computed tomography (CT) scans. On the downside FCD could be elusive in MRI images and may be practically invisible in CT scans. Furthermore, unnecessary removal of normal tissues is to be taken into consideration as this could lead to neurological defects. In this context, optical spectroscopy have been widely investigated as an alternative technique for the detection of abnormal tissues in different organ sites. Disease progression is accompanied by a number of architectural, biochemical and morphological changes. These variations are reflected in the spectral intensity and line shape. Here, in this proof of concept study we propose to investigate the application of tissue optical spectroscopy based on fluorescence excitation at two wavelength 378 and 445 nm coupled along with Raman spectroscopy for the detection of FCD on formalin fixed tissue specimens from pediatric subjects. For fluorescence at both the excitation wavelengths FCD showed a decreased intensity at longer wavelength when compared to normal tissues. Also, differences exist in the Raman spectral profiles of normal and FCD.

Paper Details

Date Published: 4 March 2016
PDF: 5 pages
Proc. SPIE 9715, Optical Diagnostics and Sensing XVI: Toward Point-of-Care Diagnostics, 97151D (4 March 2016); doi: 10.1117/12.2214649
Show Author Affiliations
Suresh Anand, European Lab. for Non-Linear Spectroscopy, Univ. of Florence (Italy)
Riccardo Cicchi, National Institute of Optics, CNR (Italy)
European Lab. for Non-Linear Spectroscopy, Univ. of Florence (Italy)
Flavio Giordano, Anna Meyer Pediatric Hospital (Italy)
Anna Maria Buccoliero, Anna Meyer Pediatric Hospital, Univ. of Florence (Italy)
Valerio Conti, Anna Meyer Pediatric Hospital, Univ. of Florence (Italy)
Renzo Guerrini, Anna Meyer Pediatric Hospital, Univ. of Florence (Italy)
Francesco Saverio Pavone, European Lab. for Non-Linear Spectroscopy, Univ. of Florence (Italy)
National Institute of Optics, CNR (Italy)


Published in SPIE Proceedings Vol. 9715:
Optical Diagnostics and Sensing XVI: Toward Point-of-Care Diagnostics
Gerard L. Coté, Editor(s)

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