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Proceedings Paper

The role of membrane dynamics in electrical and infrared neural stimulation
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Paper Abstract

We recently developed a nonlinear optical imaging technique based on second harmonic generation (SHG) to identify membrane disruption events in live cells. This technique was used to detect nanoporation in the plasma membrane following nanosecond pulsed electric field (nsPEF) exposure. It has been hypothesized that similar poration events could be induced by the thermal gradients generated by infrared (IR) laser energy. Optical pulses are a highly desirable stimulus for the nervous system, as they are capable of inhibiting and producing action potentials in a highly localized but non-contact fashion. However, the underlying mechanisms involved with infrared neural stimulation (INS) are not well understood. The ability of our method to non-invasively measure membrane structure and transmembrane potential via Two Photon Fluorescence (TPF) make it uniquely suited to neurological research. In this work, we leverage our technique to understand what role membrane structure plays during INS and contrast it with nsPEF stimulation. We begin by examining the effect of IR pulses on CHO-K1 cells before progressing to primary hippocampal neurons. The use of these two cell lines allows us to directly compare poration as a result of IR pulses to nsPEF exposure in both a neuron-derived cell line, and one likely lacking native channels sensitive to thermal stimuli.

Paper Details

Date Published: 13 May 2016
PDF: 5 pages
Proc. SPIE 9719, Biophysics, Biology, and Biophotonics: the Crossroads, 97190E (13 May 2016); doi: 10.1117/12.2214474
Show Author Affiliations
Erick K. Moen, Univ. of Southern California (United States)
Hope T. Beier, Air Force Research Lab. (United States)
Bennett L. Ibey, Air Force Research Lab. (United States)
Andrea M. Armani, Univ. of Southern California (United States)

Published in SPIE Proceedings Vol. 9719:
Biophysics, Biology, and Biophotonics: the Crossroads
Adam Wax; Vadim Backman, Editor(s)

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