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Proceedings Paper

Biodynamic profiling of three-dimensional tissue growth techniques
Author(s): Hao Sun; Dan Merrill; John Turek; David Nolte
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Paper Abstract

Three-dimensional tissue culture presents a more biologically relevant environment in which to perform drug development than conventional two-dimensional cell culture. However, obtaining high-content information from inside three dimensional tissue has presented an obstacle to rapid adoption of 3D tissue culture for pharmaceutical applications. Biodynamic imaging is a high-content three-dimensional optical imaging technology based on low-coherence interferometry and digital holography that uses intracellular dynamics as high-content image contrast. In this paper, we use biodynamic imaging to compare pharmaceutical responses to Taxol of three-dimensional multicellular spheroids grown by three different growth techniques: rotating bioreactor, hanging-drop and plate-grown spheroids. The three growth techniques have systematic variations among tissue cohesiveness and intracellular activity and consequently display different pharmacodynamics under identical drug dose conditions. The in vitro tissue cultures are also compared to ex vivo living biopsies. These results demonstrate that three-dimensional tissue cultures are not equivalent, and that drug-response studies must take into account the growth method.

Paper Details

Date Published: 17 March 2016
PDF: 14 pages
Proc. SPIE 9707, Dynamics and Fluctuations in Biomedical Photonics XIII, 97070S (17 March 2016); doi: 10.1117/12.2214309
Show Author Affiliations
Hao Sun, Purdue Univ. (United States)
Dan Merrill, Purdue Univ. (United States)
John Turek, Purdue Univ. (United States)
David Nolte, Purdue Univ. (United States)

Published in SPIE Proceedings Vol. 9707:
Dynamics and Fluctuations in Biomedical Photonics XIII
Valery V. Tuchin; Kirill V. Larin; Martin J. Leahy; Ruikang K. Wang, Editor(s)

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