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Proceedings Paper

Quantum dot based enzyme activity sensors present deviations from Michaelis-Menten kinetic model
Author(s): Sebastián A. Díaz; Carl W. Brown; Anthony P. Malanoski; Eunkeu Oh; Kimihiro Susumu; Igor L. Medintz
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Paper Abstract

Nanosensors employing quantum dots (QDs) and enzyme substrates with fluorescent moieties offer tremendous promise for disease surveillance/diagnostics and as high-throughput co-factor assays. Advantages of QDs over other nanoscaffolds include their small size and inherent photochemical properties such as size tunable fluorescence, ease in attaching functional moieties, and resistance to photobleaching. These properties make QDs excellent Förster Resonance Energy Transfer (FRET) donors; well-suited for rapid, optical measurement applications. We report enzyme sensors designed with a single FRET donor, the QD donor acting as a scaffold to multiple substrates or acceptors. The QD-sensor follows the concrete activity of the enzyme, as compared to the most common methodologies that quantify the enzyme amount or its mRNA precursor. As the sensor reports on the enzyme activity in real-time we can actively follow the kinetics of the enzyme. Though classic Michaelis-Menten (MM) parameters can be obtained to describe the activity. In the course of these experiments deviations, both decreasing and increasing the kinetics, from the common MM model were observed upon close examinations. From these observations additional experiments were undertaken to understand the varying mechanisms. Different enzymes can present different deviations depending on the chosen target, e.g. trypsin appears to present a positive hopping mechanism while collagenase demonstrates a QD caused reversible inhibition.

Paper Details

Date Published: 22 April 2016
PDF: 9 pages
Proc. SPIE 9722, Colloidal Nanoparticles for Biomedical Applications XI, 97220K (22 April 2016); doi: 10.1117/12.2214235
Show Author Affiliations
Sebastián A. Díaz, U.S. Naval Research Lab. (United States)
Carl W. Brown, U.S. Naval Research Lab. (United States)
Anthony P. Malanoski, U.S. Naval Research Lab. (United States)
Eunkeu Oh, U.S. Naval Research Lab. (United States)
Sotera Defense Solutions (United States)
Kimihiro Susumu, U.S. Naval Research Lab. (United States)
Sotera Defense Solutions (United States)
Igor L. Medintz, U.S. Naval Research Lab. (United States)

Published in SPIE Proceedings Vol. 9722:
Colloidal Nanoparticles for Biomedical Applications XI
Wolfgang J. Parak; Marek Osinski; Xing-Jie Liang, Editor(s)

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