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Proceedings Paper

Investigation of the effect of hydration on dermal collagen in ex vivo human skin tissue using second harmonic generation microscopy
Author(s): Ravikant Samatham; Nicholas K. Wang; Steven L. Jacques
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Paper Abstract

Effect of hydration on the dermal collagen structure in human skin was investigated using second harmonic generation microscopy. Dog ears from the Mohs micrographic surgery department were procured for the study. Skin samples with subject aged between 58-90 years old were used in the study. Three dimensional Multiphoton (Two-photon and backward SHG) control data was acquired from the skin samples. After the control measurement, the skin tissue was either soaked in deionized water for 2 hours (Hydration) or kept at room temperature for 2 hours (Desiccation), and SHG data was acquired. The data was normalized for changes in laser power and detector gain. The collagen signal per unit volume from the dermis was calculated. The desiccated skin tissue gave higher backward SHG compared to respective control tissue, while hydration sample gave a lower backward SHG. The collagen signal decreased with increase in hydration of the dermal collagen. Hydration affected the packing of the collagen fibrils causing a change in the backward SHG signal. In this study, the use of multiphoton microscopy to study the effect of hydration on dermal structure was demonstrated in ex vivo tissue.

Paper Details

Date Published: 29 February 2016
PDF: 10 pages
Proc. SPIE 9689, Photonic Therapeutics and Diagnostics XII, 96890W (29 February 2016); doi: 10.1117/12.2214040
Show Author Affiliations
Ravikant Samatham, Oregon Health & Science Univ. (United States)
Nicholas K. Wang, Univ. of Toronto (Canada)
Steven L. Jacques, Oregon Health & Science Univ. (United States)

Published in SPIE Proceedings Vol. 9689:
Photonic Therapeutics and Diagnostics XII
Hyun Wook Kang; Guillermo J. Tearney M.D.; Melissa C. Skala; Bernard Choi; Andreas Mandelis; Brian J. F. Wong M.D.; Justus F. Ilgner M.D.; Nikiforos Kollias; Paul J. Campagnola; Kenton W. Gregory M.D.; Laura Marcu; Haishan Zeng, Editor(s)

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