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Proceedings Paper

Watching embryonic development in a new light: elasticity specific imaging with dual Brillouin/Raman microspectroscopy
Author(s): Zhaokai Meng; Jessica A. Hanson; Vladislav V. Yakovlev
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Paper Abstract

Mechanical properties of tissues play an important role in biological development. However, the current elasticity-specific imaging techniques are either destructive / invasive, or have a limited spatial and/or temporal resolution. Recently, we introduced Brillouin microscopy imaging as a local non-invasive probe of microscopic viscoelasticity in cells and tissues. In this study, by taking advantage of Brillouin spectroscopy, we imaged the viscoelasticity properties of different compartments of living zebrafish embryos, including yolk-sac, skin, spine and heart. Brillouin and Raman spectra were collected simultaneously at each location using a recently developed Brillouin/Raman microscope.

Paper Details

Date Published: 2 May 2016
PDF: 6 pages
Proc. SPIE 9716, Optical Methods in Developmental Biology IV, 97160L (2 May 2016); doi: 10.1117/12.2213978
Show Author Affiliations
Zhaokai Meng, Texas A&M Univ. (United States)
Jessica A. Hanson, Texas A&M Univ. (United States)
Vladislav V. Yakovlev, Texas A&M Univ. (United States)

Published in SPIE Proceedings Vol. 9716:
Optical Methods in Developmental Biology IV
Andrew M. Rollins; Scott E. Fraser; Michael A. Choma, Editor(s)

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