Share Email Print

Proceedings Paper

Super resolution imaging of HER2 gene amplification
Author(s): Masaya Okada; Takuya Kubo; Kanako Masumoto; Shigeki Iwanaga
Format Member Price Non-Member Price
PDF $17.00 $21.00
cover GOOD NEWS! Your organization subscribes to the SPIE Digital Library. You may be able to download this paper for free. Check Access

Paper Abstract

HER2 positive breast cancer is currently examined by counting HER2 genes using fluorescence in situ hybridization (FISH)-stained breast carcinoma samples. In this research, two-dimensional super resolution fluorescence microscopy based on stochastic optical reconstruction microscopy (STORM), with a spatial resolution of approximately 20 nm in the lateral direction, was used to more precisely distinguish and count HER2 genes in a FISH-stained tissue section. Furthermore, by introducing double-helix point spread function (DH-PSF), an optical phase modulation technique, to super resolution microscopy, three-dimensional images were obtained of HER2 in a breast carcinoma sample approximately 4 μm thick.

Paper Details

Date Published: 12 April 2016
PDF: 5 pages
Proc. SPIE 9714, Single Molecule Spectroscopy and Superresolution Imaging IX, 97140E (12 April 2016); doi: 10.1117/12.2213918
Show Author Affiliations
Masaya Okada, Sysmex Corp. (Japan)
Takuya Kubo, Sysmex Corp. (Japan)
Kanako Masumoto, Sysmex Corp. (Japan)
Shigeki Iwanaga, Sysmex Corp. (Japan)

Published in SPIE Proceedings Vol. 9714:
Single Molecule Spectroscopy and Superresolution Imaging IX
Jörg Enderlein; Ingo Gregor; Zygmunt Karol Gryczynski; Rainer Erdmann; Felix Koberling, Editor(s)

© SPIE. Terms of Use
Back to Top