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Proceedings Paper

Super resolution imaging of HER2 gene amplification
Author(s): Masaya Okada; Takuya Kubo; Kanako Masumoto; Shigeki Iwanaga
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Paper Abstract

HER2 positive breast cancer is currently examined by counting HER2 genes using fluorescence in situ hybridization (FISH)-stained breast carcinoma samples. In this research, two-dimensional super resolution fluorescence microscopy based on stochastic optical reconstruction microscopy (STORM), with a spatial resolution of approximately 20 nm in the lateral direction, was used to more precisely distinguish and count HER2 genes in a FISH-stained tissue section. Furthermore, by introducing double-helix point spread function (DH-PSF), an optical phase modulation technique, to super resolution microscopy, three-dimensional images were obtained of HER2 in a breast carcinoma sample approximately 4 μm thick.

Paper Details

Date Published: 12 April 2016
PDF: 5 pages
Proc. SPIE 9714, Single Molecule Spectroscopy and Superresolution Imaging IX, 97140E (12 April 2016); doi: 10.1117/12.2213918
Show Author Affiliations
Masaya Okada, Sysmex Corp. (Japan)
Takuya Kubo, Sysmex Corp. (Japan)
Kanako Masumoto, Sysmex Corp. (Japan)
Shigeki Iwanaga, Sysmex Corp. (Japan)


Published in SPIE Proceedings Vol. 9714:
Single Molecule Spectroscopy and Superresolution Imaging IX
Jörg Enderlein; Ingo Gregor; Zygmunt Karol Gryczynski; Rainer Erdmann; Felix Koberling, Editor(s)

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