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Proceedings Paper

Development of an optofluidic SERS-based biomedical sensor
Author(s): Brian Walton; Po-Jung Huang; Jun Kameoka; Nicolaas Deutz; Gerard L. Coté
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Paper Abstract

Rapid assessment of radiation exposure to sensitive organs like the gut is extremely important for large populations exposed to ionized radiation, for instance during warfare. Recent results have shown that plasma citrulline levels appear to track gut function after irradiation levels in mice and humans. The current ways to monitor blood citrulline levels are bulky, laborious, time-consuming and expensive methods. Therefore, an optofludic point-of-care (POC) system using surface enhanced Raman spectroscopy to measure plasma citrulline as a marker for radiation exposure that overcomes the above issues is being developed.

As a first step toward development of this system four colloidal nanoparticles, spherical gold, silver cubes, silica-gold nanoshells, and silver-gold nanocages have been analyzed for use in the POC system. Transmission electron microscopy (TEM) images have been taken of each nanoparticle to visualize the morphology of the nanoparticles, which is vital for SERS. Ultraviolet-visible (UV/Vis) spectroscopy was also collected to verify the extinction spectra for each nanoparticle was in resonance with the excitation wavelength. The nanoparticles were functionalized with mercaptobenzoic acid (MBA), a Raman reporter molecule, and SERS spectra were collected to determine which has better utility in a novel micro-to-nanochannel. The data showed that the silver nanocubes have a larger enhancement factor than the gold nanospheres, nanoshells, or nanocages. Currently, these nanocubes are being functionalized with the citulline for assessing the concentration sensitivity and dynamic range for ultimate use as a marker for radiation.

Paper Details

Date Published: 4 March 2016
PDF: 7 pages
Proc. SPIE 9715, Optical Diagnostics and Sensing XVI: Toward Point-of-Care Diagnostics, 97150D (4 March 2016); doi: 10.1117/12.2213909
Show Author Affiliations
Brian Walton, Texas A&M Univ. (United States)
Po-Jung Huang, Texas A&M Univ. (United States)
Jun Kameoka, Texas A&M Univ. (United States)
Nicolaas Deutz, Texas A&M Univ. (United States)
Gerard L. Coté, Texas A&M Univ. (United States)

Published in SPIE Proceedings Vol. 9715:
Optical Diagnostics and Sensing XVI: Toward Point-of-Care Diagnostics
Gerard L. Coté, Editor(s)

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