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Proceedings Paper

Optogenetic pacing in Drosophila melanogaster (Conference Presentation)
Author(s): Aneesh Alex; Airong Li; Jing Men; Jason Jerwick; Rudolph E. Tanzi; Chao Zhou; Xianxu Zeng; Zhan Zhang
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Paper Abstract

A non-invasive, contact-less cardiac pacing technology can be a powerful tool in basic cardiac research and in clinics. Currently, electrical pacing is the gold standard for cardiac pacing. Although highly effective in controlling the cardiac function, the invasive nature, non-specificity to cardiac tissues and possible tissue damage limits its capabilities. Optical pacing of heart is a promising alternative, which is non-invasive and more specific, has high spatial and temporal precision, and avoids shortcomings in electrical stimulation. Optical coherence tomography has been proved to be an effective technique in non-invasive imaging in vivo with ultrahigh resolution and imaging speed. In the last several years, non-invasive specific optical pacing in animal hearts has been reported in quail, zebrafish, and rabbit models. However, Drosophila Melanogaster, which is a significant model with orthologs of 75% of human disease genes, has rarely been studied concerning their optical pacing in heart. Here, we combined optogenetic control of Drosophila heartbeat with optical coherence microscopy (OCM) technique for the first time. The light-gated cation channel, channelrhodopsin-2 (ChR2) was specifically expressed by transgene as a pacemaker in drosophila heart. By stimulating the pacemaker with 472 nm pulsed laser light at different frequencies, we achieved non-invasive and more specific optical control of the Drosophila heart rhythm, which demonstrates the wide potential of optical pacing for studying cardiac dynamics and development. Imaging capability of our customized OCM system was also involved to observe the pacing effect visually. No tissue damage was found after long exposure to laser pulses, which proved the safety of optogenetic control of Drosophila heart.

Paper Details

Date Published: 26 April 2016
PDF: 1 pages
Proc. SPIE 9690, Clinical and Translational Neurophotonics; Neural Imaging and Sensing; and Optogenetics and Optical Manipulation, 96902F (26 April 2016); doi: 10.1117/12.2213877
Show Author Affiliations
Aneesh Alex, Lehigh Univ. (United States)
Airong Li, Massachusetts General Hospital (United States)
Jing Men, Lehigh Univ. (United States)
Jason Jerwick, Lehigh Univ. (United States)
Rudolph E. Tanzi, Massachusetts General Hospital (United States)
Chao Zhou, Lehigh Univ. (United States)
Xianxu Zeng, Lehigh Univ. (United States)
Zhan Zhang, Third Affiliated Hospital of Zhengzhou Univ. (China)


Published in SPIE Proceedings Vol. 9690:
Clinical and Translational Neurophotonics; Neural Imaging and Sensing; and Optogenetics and Optical Manipulation
Steen J. Madsen; E. Duco Jansen; Samarendra K. Mohanty; Nitish V. Thakor; Qingming Luo; Victor X. D. Yang, Editor(s)

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